10x
Support
Search Support
Contact us
10x Genomics Support/

Spatial Gene Expression for FFPE

product-intro
What is Spatial Gene Expression for FFPE?

Visium Spatial Gene Expression measures the whole transcriptome of FFPE tissue sections in a spatially resolved manner by mapping gene expression over a high-resolution microscope image of the H&E- or IF-stained tissue.

Get startedFind User Guides

Documentation

Find resources, from protocols to free-to-use analysis tools.

Experimental Planning & Design

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial Gene Expression for FFPE protocols.

Tissue Prep

Best practices for handling tissue samples and slides before and after sectioning. Guidance for removing hard coverslips from archived tissue slides.

Tissue Staining

Deparaffinize, stain, image, and decrosslink tissue.

Probe Sets

Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium for FFPE assay.

Library Construction

Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.

Instruments

Refer to instrument-specific documents for seamless instrument operation.

Imaging

For imaging hardware, general image acquisition and analysis guidelines, and example images.

Sequencing

Sequence your Visium spatial library as per our provided recommendations.

Software Analysis

Analyze your Visium spatial data using our pipelines and visualization tools.

Datasets

Access free, publicly available data from a number of different sample types generated with Visium for FFPE spatial solutions

View datasets
Datasets

Videos - Visium CytAssist Spatial Gene Expression for FFPE

Visium CytAssist Instrument Tour and Readiness Test video
How-to series
Visium CytAssist Instrument Tour and Readiness Test
Tissue Preparation Guide video
Demonstrated Protocol series
Tissue Preparation Guide
Deparaffinization, H&E Staining, Imaging & Decrosslinking video
How-to series
Deparaffinization, H&E Staining, Imaging & Decrosslinking
View all videos
Q&A

Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.

Q&A
Publications

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.

Publications