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10x Genomics Support/Spatial Gene Expression for FFPE/


Find resources from protocols to free-to-use analysis tools.
Experimental Planning & Design

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial Gene Expression for FFPE protocols.

Tissue Prep

Best practices for handling tissue samples and slides before and after sectioning. Guidance for removing hard coverslips from archived tissue slides.

Tissue Staining

Deparaffinize, stain, image, and decrosslink tissue.

Probe Sets

Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium for FFPE assay.

Library Construction

Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.


Refer to instrument-specific documents for seamless instrument operation.


For imaging hardware, general image acquisition and analysis guidelines, and example images.


Sequence your Visium spatial library as per our provided recommendations.

Software Analysis

Analyze your Visium spatial data using our pipelines and visualization tools.