Cell surface proteins can be labeled using a specific protein binding molecule, such as an antibody conjugated to a Feature Barcode oligonucleotide. This protocol outlines cell surface protein labeling for use with Fixed RNA Profiling with Feature Barcode technology for Cell Surface Protein. This protocol also provides guidance for enriching labeled cells using Fluorescence Activated Cell Sorting (FACS).
Optional cell surface protein labeling must be completed prior to sample fixation.
TotalSeq™️-C antibodies are compatible with Singleplex and Multiplex workflows (via Barcode Oligo Capture); TotalSeq™️-B antibodies are only compatible with Singleplex workflow.
- Document Type
- Demonstrated Protocol
- Last Modified
- November 28, 2023