Spatial Gene and Protein Expression
What is Visium CytAssist Spatial Gene and Protein Expression?
Visium CytAssist Spatial Gene and Protein Expression simultaneously measures whole transcriptome and highly multiplexed protein expression of FFPE tissue sections in a spatially resolved manner by mapping gene and protein expression over a high-resolution microscope image of the H&E- or IF-stained tissue.
Find resources, from protocols to free-to-use analysis tools. Visium CytAssist Spatial Gene and Protein Expression currently supports immune cell-containing human FFPE samples.
Plan ahead by referring to the list of equipment, kits, and reagents needed for the Spatial Gene and Protein Expression protocols
For proper FFPE tissue preparation
Deparaffinize, decrosslink, and stain tissue
For imaging hardware, general image acquisition and analysis guidelines, and example images
Find a list of probes targeting protein-coding genes in human transcriptome for the Spatial Gene and Protein Expression assay
Find a list of proteins detected by the human protein panel for the Spatial Gene and Protein Expression assay
Construct sequencing-ready Visium spatial libraries from your FFPE tissue sections
Refer to instrument-specific documents for seamless instrument operation
Sequence your Spatial Gene and Protein Expression libraries as per our provided recommendations
Analyze your Visium spatial data using our pipelines and visualization tools.