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CytAssist Spatial Gene and Protein Expression

CytAssist Spatial Gene and Protein Expression

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What is Visium CytAssist Spatial Gene and Protein Expression?

Visium CytAssist Spatial Gene and Protein Expression simultaneously measures whole transcriptome and highly multiplexed protein expression of FFPE tissue sections in a spatially resolved manner by mapping gene and protein expression over a high-resolution microscope image of the H&E- or IF-stained tissue.

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Documentation

Find resources, from protocols to free-to-use analysis tools. Visium CytAssist Spatial Gene and Protein Expression currently supports immune cell-containing human FFPE samples.

Experimental Planning & Design

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Spatial Gene and Protein Expression protocols

Tissue Prep

For proper FFPE tissue preparation

Tissue Staining

Deparaffinize, decrosslink, and stain tissue

Imaging

For imaging hardware, general image acquisition and analysis guidelines, and example images

Probe Set

Find a list of probes targeting protein-coding genes in human transcriptome for the Spatial Gene and Protein Expression assay

Protein Panel

Find a list of proteins detected by the human protein panel for the Spatial Gene and Protein Expression assay

Library Construction

Construct sequencing-ready Visium spatial libraries from your FFPE tissue sections

Instruments

Refer to instrument-specific documents for seamless instrument operation

Sequencing

Sequence your Spatial Gene and Protein Expression libraries as per our provided recommendations

Software Analysis

Analyze your Visium spatial data using our pipelines and visualization tools.

Datasets

Official datasets are coming soon. Access free, publicly available data from a number of different sample types generated with Visium Spatial solutions.

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Datasets

Videos - CytAssist Spatial Gene and Protein Expression

Visium CytAssist Instrument video
How-to series
Visium CytAssist Instrument
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Q&A

Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.

Q&A