10x Genomics Support/Visium CytAssist Spatial Gene and Protein Expression for FFPE/

Documentation

Find resources from protocols to free-to-use analysis tools.
Experimental Planning & Design

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Spatial Gene and Protein Expression protocols

Tissue Prep

For proper FFPE tissue preparation

Tissue Staining

Deparaffinize, decrosslink, and stain tissue

Imaging

For imaging hardware, general image acquisition and analysis guidelines, and example images

Probe Set

Find a list of probes targeting protein-coding genes in human transcriptome for the Spatial Gene and Protein Expression assay

Protein Panel

Find a list of proteins detected by the human protein panel for the Spatial Gene and Protein Expression assay

Library Construction

Construct sequencing-ready Visium spatial libraries from your FFPE tissue sections

Instruments

Refer to instrument-specific documents for seamless instrument operation

Sequencing

Sequence your Spatial Gene and Protein Expression libraries as per our provided recommendations

Software Analysis

Analyze your Visium spatial data using our pipelines and visualization tools.