Tested Tissue List
List of tissue types that were tested by 10x and found to perform well with the Visium CytAssist Spatial Gene Expression for FFPE assay. Tissues tested in-house were acquired from biobanks. Please note: sample preparation variability (such as fixation and embedding techniques) may impact assay performance.
Datasets from a subset of tested tissues are available here.
| Organism | Tissue | Unknown, Healthy, Diseased (Region, if known) | Important Considerations |
|---|---|---|---|
| Human | Brain | Healthy (Cortex) | |
| Human | Brain | Diseased, Glioblastoma | |
| Human | Breast | Diseased, Cancer | |
| Human | Kidney | Healthy | |
| Human | Large Intestine | Diseased, Colorectal Cancer | |
| Human | Liver | Diseased, Hepatocellular Carcinoma | |
| Human | Lung | Diseased, Cancer | |
| Human | Lymph Node | Healthy | |
| Human | Ovary | Diseased, Cancer | |
| Human | Pancreas | Diseased, Invasive Adenocarcinoma | |
| Human | Pancreas | Diseased, Metastatic Pancreatic Ductal Adenocarcinoma (Resection of splenic metastasis) | |
| Human | Prostate | Diseased, Cancer | |
| Human | Skin | Diseased, Melanoma | |
| Human | Thymus | Healthy | |
| Human | Tonsil | Diseased, Tonsilitis | |
| Human | Tonsil | Diseased, Follicular Hyperplasia | |
| Human (TMA) | Breast (11 x 6 Array, 1 mm Diameter, 1 mm Edge-to-Edge Distance) | Cancer | Expertise required for generating TMA blocks, sectioning and tissue placement. See Q&A article for further details. |
| Mouse | Brain | Healthy | |
| Mouse | Embryo | Healthy | |
| Mouse | Heart | Healthy | |
| Mouse | Kidney | Healthy | |
| Mouse | Liver | Healthy | |
| Mouse | Small Intestine | Healthy | |
| Mouse | Spleen | Healthy | |
| Mouse | Testes | Healthy | |
| Mouse | Thymus | Healthy | |
| Mouse (TMA) | Brain & Kidney (5 x 5 Array, 1 mm Diameter, 1 mm Edge-to-Edge Distance) | Healthy | Expertise required for generating TMA blocks, sectioning and tissue placement. See Q&A article for further details. |
| Mouse (TMA) | Brain & Lung (3 x 3 Arrays, 2 mm Diameter, 1 or 2 mm Edge-to-Edge Distance) | Healthy | Expertise required for generating TMA blocks, sectioning and tissue placement. See Q&A article for further details. |
Challenging Tissues
List of tissue types tested by 10x with the Visium CytAssist Spatial Gene Expression for FFPE assay that were found to not perform well with 10x protocols. Notes on why these samples were challenging are listed.
| Organism | Tissue | Unknown, Healthy, Diseased (Region, if known) | Additional Information |
|---|---|---|---|
| Human | Bone | Healthy (Femur) | Observed a high rate of tissue detachment, high percentage of UMI reads from gDNA, and low sensitivity. See Q&A article for further details. |
| Human | Heart | Healthy | Observed low sensitivity and poor clustering with this tissue type. |
| Human | Skin | Healthy | Observed a high rate of tissue detachment, high percentage of UMI reads from gDNA and low sensitivity. See Q&A article for further details. |
| Human | Spleen | Healthy | Observed a high percentage of UMI reads from gDNA which varied depending upon tissue block. |
| Mouse | Bone | Healthy (Femur), Healthy (Tibia) | Observed a high rate of tissue detachment and low sensitivity. See Q&A article for further details. |
| Mouse | Skin | Healthy | Observed low sensitivity. See Q&A article for further details. |