The Visium CytAssist Spatial Gene Expression workflow assays RNA levels in intact formalin-fixed paraffin embedded (FFPE), Fresh Frozen (FF), or Fixed Frozen (FxF) tissue sections. It uses probes targeting the whole transcriptome. Each Visium CytAssist Spatial Gene Expression Slide contains Capture Areas with barcoded spots that include oligonucleotides required to capture gene expression probes.

This document outlines the protocol for generating Visium CytAssist Spatial Gene Expression probe-based libraries from FFPE, FF, or FxF tissue sections.

For use with:

• Visium CytAssist Spatial Gene Expression for FFPE, Human Transcriptome, 6.5 mm, 4 rxns PN-1000520
• Visium CytAssist Spatial Gene Expression for FFPE, Mouse Transcriptome, 6.5 mm, 4 rxns PN-1000521
• Visium CytAssist Spatial Gene Expression for FFPE, Human Transcriptome, 11 mm, 2 rxns PN-1000522
• Visium CytAssist Spatial Gene Expression for FFPE, Mouse Transcriptome, 11 mm, 2 rxns PN-1000523
• Visium CytAssist Reagent Accessory Kit, PN-1000499
• Dual Index Kit TS Set A, 96 rxns PN-1000251