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Lists all the documents available for sample preparation and library construction for Visium Spatial for FFPE.

Visium Spatial Gene Expression for FFPE Document Resources

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial Gene Expression for FFPE protocols.

Experimental Design

Experimental Planning and Design

Visium Spatial Gene Expression measures the whole transcriptome of FFPE tissue sections in a spatially resolved manner by mapping gene expression over a high-resolution microscope image of the H&E- or IF-stained tissue.

CytAssist for FFPE

Experimental Planning & Design

Best practices for handling tissue samples and slides before and after sectioning. Guidance for removing hard coverslips from archived tissue slides.

Tissue Prep

Deparaffinize, stain, image, and decrosslink tissue.

Tissue Staining

Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium for FFPE assay.

Probe Sets

Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.

Library Construction

Refer to instrument-specific documents for seamless instrument operation.

Instruments

For imaging hardware, general image acquisition and analysis guidelines, and example images.

Imaging

Sequence your Visium spatial library as per our provided recommendations.

Sequencing

Analyze your Visium spatial data using our pipelines and visualization tools.

Software Analysis

CytAssist Spatial Gene Expression for FFPE

FFPE

For proper FFPE tissue sectioning and placement.

Spatial Gene Expression for FFPE

Visium CytAssist

Spatial Gene Expression

Overview

Documentation

Datasets

Videos

Publications

What is Spatial Gene Expression for FFPE?

Find resources, from protocols to free-to-use analysis tools.

Access free, publicly available data from a number of different sample types generated with Visium for FFPE spatial solutions

Videos for Visium CytAssist for FFPE are coming soon.

Visium for FFPE Gene Expression Protocol

Visium for FFPE Tissue Preparation Guide

Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.

Visium Spatial Gene Expression for FFPE Document Resources

Getting Started, CG000483

CG000483_Document Resources_VisiumSpatialProtocols_FFPE_Rev A.pdf