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10x Genomics Support/Spatial Gene Expression for FFPE/Documentation/Spatial Gene Expression for FFPE v1/Experimental Planning and Design/

Visium Spatial Gene Expression for FFPE Tested Tissues

Tested Tissue List

* List of tissue types that were tested in-house and found to perform well with Visium for FFPE. Tissues tested in-house were acquired from biobanks. Please note: sample preparation variability (such as fixation and embedding techniques) may impact assay performance.

OrganismTissueUnknown, Healthy, DiseasedRegion, if known
HumanBrainUnknownCerebellum
HumanBrainHealthy
HumanBrainDiseased, Glioblastoma
HumanBreastHealthy
HumanBreastDiseased, Cancer
HumanCervixDiseased, Cancer
HumanIntestineDiseased, Colorectal Cancer
HumanSpineDiseased, Glioblastoma
HumanHeartHealthy
HumanHeartDiseased, Myxoma
HumanHeartDiseased, Sarcoma
HumanKidneyHealthy
HumanLarge IntestineHealthy
HumanLarge IntestineDiseased, Cancer
HumanLungHealthy
HumanLungDiseased, Cancer
HumanLymph NodeHealthy
HumanLymph NodeDiseased, Inflamed
HumanLymph NodeDiseased, Lymphoma
HumanOvaryDiseased, Cancer
HumanProstateHealthy
HumanProstateDiseased, Cancer
HumanSkinDiseased, Malignant Melanoma
HumanSkinDiseased, Squamous Cell Carcinoma
HumanSkinDiseased, Merkel Cell Carcinoma
HumanSpinal CordUnknown
HumanSpleenHealthy
MouseBrainHealthy
MouseBrainHealthyCerebellum
MouseBrainHealthyOlfactory bulb
MouseHeartHealthy
MouseKidneyHealthy
MouseLiverHealthy
MouseSpleenHealthy
MouseTestisHealthy
MouseThymusHealthy
MouseEmbryoHealthy
MouseEyeHealthy
MouseMuscleHealthyQuadricep
MouseOvaryHealthy
MousePlacentaHealthyEmbryonic (E17)
MouseSmall IntestineHealthy
MouseSkinHealthy
MouseTongueHealthy

Challenging Tissues

* List of tissue types tested in-house with Visium for FFPE that were found to not perform well. Notes on why these samples were challenging are listed.

OrganismTissueUnknown, Healthy, DiseasedAdditional Information
HumanAdiposeUnknownLow yield / Poor sensitivity
HumanTonsilDiseased, Reactive Follicular HyperplasiaInconsistent permeabilization efficiency
HumanSkinNormalHigh propensity for detachment
MouseBone/CartilageHealthyRequires further optimization
Document Type
Specifications

Last Modified
January 20, 2022