This assay requires CytAssist instrument Firmware v2.0.0 or higher. Refer to the Visium CytAssist with Readiness Test User Guide (CG000542) for details.
This document outlines the protocol for generating Visium HD Spatial Gene Expression libraries from FFPE tissue sections. Key companion documents for use with this document:
- Visium HD Spatial Applications Protocol Planner (CG000698)
- Tissue Slide Alignment Instruction, Quick Reference Cards (CG000648)
- Visium Cassette S3 for Assembly and Disassembly Quick Reference Cards (CG000730)
For use with:
- Visium HD, Human Transcriptome, 6.5 mm, 4 rxns, PN-1000675
- Visium HD, Human Transcriptome, 6.5 mm, 16 rxns, PN-1000673
- Visium HD, Mouse Transcriptome, 6.5 mm, 4 rxns, PN -1000676
- Visium HD, Mouse Transcriptome, 6.5 mm, 16 rxns, PN-1000674
- Visium CytAssist Reagent Accessory Kit, PN-1000499
- Dual Index Kit TS Set A, 96 rxns, PN-1000251