Visium HD FFPE Tissue Preparation Handbook 2.0 is compatible with 6.5mm and 11mm slide workflow. If the original protocol is required for use, consult Visium HD FFPE Tissue Preparation Handbook (CG000684)
This document provides best practices for handling FFPE tissue samples and generating tissue slides. Protocols for performing deparaffinization, staining (H&E and IF), and decrosslinking of tissue sections. Key companion documents for use with this document:
- Visium HD Spatial Gene Expression Protocol Planner (CG000698)
- Tissue Slide Alignment Instruction, Quick Reference Cards (CG000548)
- Visium Cassette S3 for Assembly & Disassembly (CG000730)
For use with:
- Visium HD, Human Transcriptome, 6.5 mm, PN-1000675 (4 rxns) / PN-1000673 (16 rxns)
- Visium HD, Mouse Transcriptome, 6.5 mm, PN -1000676 (4 rxns) / PN-1000674 (16 rxns)
- Visium HD, Human Transcriptome, 11 mm, PN-1001037 (2 rxns) / PN-1001039 (8 rxns)
- Visium HD, Mouse Transcriptome, 11 mm, PN -1001038 (2 rxns) / PN-1001040 (8 rxns)
- Visium CytAssist Reagent Accessory Kit, PN-1000499
- Dual Index Kit TS Set A, 96 rxns, PN-1000251