This document provides best practices for handling FFPE tissue samples and generating tissue slides. Protocols for performing deparaffinization, staining (H&E and IF), and decrosslinking of tissue sections. Key companion documents for use with this document:

1. Visium HD Spatial Applications Protocol Planner (CG000698)
2. Tissue Slide Alignment Instruction, Quick Reference Cards (CG000648)
3. Visium Cassette S3 for Assembly & Disassembly (CG000730)

For use with:

• Visium HD, Human Transcriptome, 6.5 mm, 4 rxns, PN-1000675
• Visium HD, Human Transcriptome, 6.5 mm, 16 rxns, PN-1000673
• Visium HD, Mouse Transcriptome, 6.5 mm, 4 rxns, PN-1000676
• Visium HD, Mouse Transcriptome, 6.5 mm, 16 rxns, PN-1000674
• Visium CytAssist Reagent Accessory Kit, PN-1000499
• Dual Index Kit TS Set A, 96 rxns, PN-1000251