Human Breast Cancer: Targeted, Immunology Panel
Spatial Gene Expression dataset analyzed using Space Ranger 1.2.0
![](https://cdn.10xgenomics.com/image/upload/v1718063354/datasets/detail/visium.png)
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10X Genomics obtained fresh frozen human Invasive Lobular Carcinoma breast tissue from BioIVT Asterand. The tissue was embedded and cryosectioned as described in Visium Spatial Protocols – Tissue Preparation Guide Demonstrated Protocol (CG000240). Tissue sections of 10µm were placed on Visium Gene Expression slides and fixed and stained following Methanol Fixation, H&E Staining & Imaging for Visium Spatial Protocols (CG000160).
The tissue was AJCC/UICC Stage Group I, ER positive, PR positive, HER2 negative.
The H&E image was acquired using a Nikon Eclipse Ti2-E microscope with the following settings:
- Color camera
- 10X objective
- Numerical Aperture: 0.45
- Exposure: 20 ms
The Visium Gene Expression library (T1T2-A6) was prepared as described in the Visium Spatial Reagent Kits User Guide (CG000239 Rev D). Targeted libraries were prepared following the Targeted Gene Expression - Spatial User Guide (CG000377) using the Human Immunology Panel (PN-2000286). Targeted sequencing data was processed using Space Ranger.
- Sequencing instrument: Illumina NovaSeq 6000, flow cell HHWHLDSXY (lane 4)
- Sequencing depth: 16,141 mean reads per cell
- Sequencing configuration: Paired-end (28 X 90), Dual-Indexed Sequencing. Read 1: 28 cycles (16 bp barcode, 12 bp UMI); i7 index: 10 cycles; i5 index: 10 cycles; Read 2: 90 cycles (transcript).
- Slide: V19L29-095
- Area: A1
Key targeted metrics were:
- Spots detected: 4,325
- Median targeted genes per spot: 203
- Median targeted UMI counts per spot: 672
- Reads mapped confidently to the targeted transcriptome: 84.2%
- Total targeted genes detected: 973
To maintain donor anonymity, sequence data for this sample is not currently available.
This dataset is licensed under the Creative Commons Attribution license.