Prepare single cell/nuclei suspension from fresh samples for fixation or from archived samples by following our Demonstrated Protocols.

Sample Prep

### What is Fixed RNA Profiling with Protein Labeling?

* Reveal gene expression and cell surface protein from the same fixed cell
* [To learn more click here](/products/single-cell-gene-expression-flex)
* Compatible with Singleplex or Multiplex Samples via Direct Capture (TotalSeq™️-B) or Barcode Oligo Capture (TotalSeq™️-C)

### Fixed RNA Profiling Feature Barcode Compatibility Table

| Fixed RNA Profiling Plexy | Direct Capture | Barcode Oligo Capture | Antibody Compatibility | Compatible Feature Barcode Kit                          |
| ------------------------- | -------------- | --------------------- | ---------------------- | ------------------------------------------------------- |
| Singleplex                | <Check />      |                       | TotalSeq™️-B           | Fixed RNA Feature Barcode Kit (PN-1000419)              |
| Singleplex                |                | <Check />             | TotalSeq™️-C           | Fixed RNA Feature Barcode Multiplexing Kit (PN-1000628) |
| Multiplex                 |                | <Check />             | TotalSeq™️-C           | Fixed RNA Feature Barcode Multiplexing Kit (PN-1000628) |

Reveal gene expression and protein expression from the same fixed cell.

Fixed RNA Profiling with Cell Surface Protein Labeling

Protein

It is critical to choose the User Guide / Protocol that corresponds to your Reagent Kit version. Find the right User Guide via the button below.

Additional Applications

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Gene Expression Flex.

Experimental Design

Experimental Design & Planning

Find a list of probes targeting protein-coding genes in human and mouse transcriptomes for the Single Cell Gene Expression Flex assay.

Probe Sets

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Sequence your genome and exome library as per our provided recommendations.

Sequencing

Analyze your genome and exome data using our pipelines and visualization tools.