This protocol outlines fixation of blood and isolation of peripheral blood mononuclear cells (PBMCs) and leukocytes from the fixed blood for use with the Chromium Fixed RNA Profiling workflow.

Blood Fixation and Cell Isolation for Chromium Fixed RNA Profiling

Prepare single cell/nuclei suspension from fresh samples for fixation or from archived samples by following our Demonstrated Protocols.

Sample Prep

Single Cell Gene Expression Flex enables single cell gene expression on precious samples that were previously inaccessible due to logistical challenges in sample handling. Fixation at the point of sample collection preserves fragile biology and greatly streamlines workflows to provide critical insights on more samples.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Gene Expression Flex.

Experimental Design

Experimental Design & Planning

Find a list of probes targeting protein-coding genes in human and mouse transcriptomes for the Single Cell Gene Expression Flex assay.

Probe Sets

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Sequence your genome and exome library as per our provided recommendations.

Sequencing

Analyze your genome and exome data using our pipelines and visualization tools.

Software Analysis

Chromium X Series

Single Cell Gene Expression Flex

Overview

Documentation

Additional Applications

Datasets

Videos

<Flex gap={'1rem'}><Text as={'span'} size={'medium'} weight={'medium'}>{`Important Workflow Update for Loading Concentration and Annealing Temperature`}</Text><Anchor href={'https://cdn.10xgenomics.com/image/upload/v1699311359/support-documents/10x_CG000716_RevA_Workflow-Update_FLEX_Loading-concentration-NovaSeq-6000_Pre-Amp-annealing-temp_Letter_digital.pdf'}><Text as={'span'} color={'inherit'} size={'medium'} weight={'medium'}>{'Learn more'}</Text></Anchor></Flex>

What is Single Cell Gene Expression Flex?

Reveal gene expression and protein expression from the same fixed cell.

Protein

We provide resources from protocols, user guides, and free-to-use analysis tools.

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

Chromium Next GEM Single Cell Gene Expression Flex

Chromium Next GEM Tissue Fixation and Dissociation

Chromium Next GEM Single Cell Isolation from FFPE Sections

Find answers to common technical questions, from sample prep through data analysis, about the Single Cell Gene Expression Flex workflow.

Blood Fixation and Cell Isolation for Chromium Fixed RNA Profiling

Demonstrated Protocol, CG000721

CG000721_DemonstratedProtocol_BloodFixation_Cell_Isolation_FixedRNAProfiling_RevB.pdf