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10x Genomics Support/Single Cell Gene Expression/Documentation/Experimental Design & Planning/

Getting Started: Single Cell 3’ Gene Expression

  • CG000360_GettingStartedSingleCell3'GeneExpression_RevA.pdf

    Getting Started, CG000360

    CG000360_GettingStartedSingleCell3'GeneExpression_RevA.pdf

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The Chromium Single Cell Gene Expression Solution provides a scalable microfluidic platform for gene expression profiling of 500-10,000 individual cells per sample. It is done by partitioning thousands of cells into nanoliter-scale Gel Beads-in-emulsion (GEMs), where all generated cDNA molecules from an individual GEM share a common 10x Barcode. Sequencing-ready libraries are generated and sequenced, and the unique 10x barcodes are used to associate reads back to the individual partitions. This document provides guidance on how to get started with the Single Cell Gene Expression assay.

FOR USE WITH:

  • Chromium Single Cell 3’ Reagent Kits (v3, v3.1, v3.1 Dual Index)
Document Type
Getting Started

Last Modified
August 9, 2021