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Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing

  • CG00055_Demonstrated_Protocol_Dissociation_Mouse_Neural_Tissue_Rev C.pdf

    Demonstrated Protocol, CG00055

    CG00055_Demonstrated_Protocol_Dissociation_Mouse_Neural_Tissue_Rev C.pdf

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  • 20191126_CG00055_Dissociation_Mouse_Neural_Tissue_RevBtoRevC.pdf

    Demonstrated Protocol, CG00055

    20191126_CG00055_Dissociation_Mouse_Neural_Tissue_RevBtoRevC.pdf

    View and download

This protocol outlines how to obtain a single cell suspension from embryonic mouse brain tissue for use with 10x Genomics Single Cell protocols. The surgical dissection of embryonic mouse tissue is not described here.

Important
This demonstrated protocol is optimized for counting cells in the range of 700-1200 cells/µl. If using the Single Cell 3' LT v3.1 (low throughput) application, ensure cells are counted as indicated in this protocol and then diluted to the LT specific optimal loading concentration of 100-600 cells/µl using the Cell Dilution Overview in the LT User Guide.
Document Type
Demonstrated Protocol

Last Modified
July 12, 2022