This Demonstrated Protocol outlines how to obtain a single protoplast suspension from the spreading earthmoss, Physcomitrella patens, (Grandsen or Villesexel ecotypes) in preparation for use in 10x Genomics® Single Cell Protocols. The protoplasts are “naked” plant cells without the cell wall but with an intact plasma membrane.
 | This demonstrated protocol is optimized for use with standard throughput 10x assays. If using the Single Cell 3’ LT v 3.1 (low throughput) application, diluting cells to the LT specific optimal loading concentrations of 100-600 cells/µl using the resuspension buffer/media can impact assay performance. |