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Single Cell Gene Expression Demonstrated Protocol Compatibility Table

ProtocolSC3'v3/v3.1SC3'v3/v3.1 with CRISPR ScreeningSC3'v3/v3.1 with Cell Surface ProteinSC3’v3.1 with Cell Multiplexing
Single Cell Protocols - Cell Preparation Guide
Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing✓*-
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling✓*-✓*✓*
Tumor Dissociation for Single Cell RNA Sequencing✓*-✓*
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing✓*✓*✓*✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing✓*-
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing-
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing-
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing-✓*
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols--
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing-✓*
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols-

✓ Compatible (tested in-house) ✓* Untested but low risk ** Compatible but unsupported - Untested, use at your own risk ✘ Incompatible

Document Type
Demonstrated Protocol

Last Modified
June 17, 2022