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Single Cell Gene Expression HT Demonstrated Protocol Compatibility Table

ProtocolHTHT with CRISPR ScreeningHT with Cell Surface ProteinHT with Cell MultiplexingHT with CRISPR Screening & Cell MultiplexingHT with Cell Surface Protein & Cell Multiplexing
Single Cell Protocols - Cell Preparation Guide
Methanol Fixation of Cells for Single Cell RNA Sequencing-
Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing✓*
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling✓*-✓*✓*-✓*
Tumor Dissociation for Single Cell RNA Sequencing--
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing✓*✓*✓*✓*✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing------
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing--
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing--
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols---
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing✓*✓*✓*✓*✓*✓*
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols---
High Throughput Sample Preparation for Single Cell RNA Sequencing-----

✓ Compatible (tested in-house) ✓* Untested but low risk ** Compatible but unsupported - Untested, use at your own risk ✘ Incompatible

Document Type
Demonstrated Protocol

Last Modified
June 17, 2022