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Sequencing Metrics & Base Composition of Visium Spatial Gene Expression Libraries

  • CG000290_TechNote_SequencingMetrics&BaseCompofVisiumSpatialGeneExpressionLibraries_RevB.pdf

    Technical Note, CG000290

    CG000290_TechNote_SequencingMetrics&BaseCompofVisiumSpatialGeneExpressionLibraries_RevB.pdf

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The Visium Spatial Gene Expression Reagent Kits workflow produces Visium Spatial Gene Expression libraries for measuring total mRNA in intact tissue sections. This Technical Note presents a comparison of sequencing metrics for pooled Visium Spatial Gene Expression libraries across Illumina️ platforms. The expected base percentage profiles and Phred quality scores based on a control library are described to provide general guidance on the expected range of sequencing metrics on Illumina platforms. Individual results may vary depending on the specific sequencing instrument and/or particular sample and loading characteristics.

FOR USE WITH:

  • Visium Spatial Gene Expression Slide & Reagent Kit, 16 rxns PN-1000184
  • Visium Spatial Gene Expression Slide & Reagent Kit, 4 rxns PN-1000187
  • Visium Gateway Gene Expression Slide & Reagent Kit, 2 rxns PN-1000317
  • Visium Accessory Kit, PN-1000194
  • Dual Index Kit TT Set A, 96 rxns PN-1000215
Document Type
Technical Note

Last Modified
March 15, 2022