HD WT Panel Gene Expression
Recommended Visium assay for differential gene expression in human and mouse
Single cell–scale resolution
Why choose HD WT Panel Gene Expression
Broad tissue compatibility including FFPE, fresh frozen, and fixed frozen
Most sequencing-efficient Visium HD assay
Assay specifications
Mouse
Human
Species
FFPE tissue
Fresh frozen tissue
Fixed frozen tissue
Compatible samples
Probe-based gene expression
Protein
Morphology
Multiomics
Compatible instruments
Throughput options
Capture Area size: 6.5 x 6.5 mm
Assay components
Consumables
Index Kit
1000251
Dual Index Kit TS Set A, 96 rxn
Core Slides and Reagents
1000675
Visium HD, Human Transcriptome, 6.5 mm, 4 rxns
1000673
Visium HD, Human Transcriptome, 6.5 mm, 16 rxns
1000676
Visium HD, Mouse Transcriptome, 6.5 mm, 4 rxns
1000674
Visium HD, Mouse Transcriptome, 6.5 mm, 16 rxns
Starter Kits and Accessories
1000685
Visium 8-port Cassette S3, 4 pk
1000684
Visium Tissue Slide Cassette S3, 6.5 mm, 4 pk
Optional Accessories
1000886
Visium CytAssist Alignment Aid Kit, 6.5 mm
RNase Inhibitor (Optional)
1000887
Kit, RNase Inhibitor 40x
Documents and resources
User guides
Sample prep
Technical notes
Sequencing requirements
The final gene expression library is sequenced at a recommended minimum depth of 275 million read pairs for Capture Areas fully covered by tissue.
Analysis guides
Resources
Fixed and fresh frozen launch webinar
Your introduction to Visium HD: Spatial biology in high definition
Getting started with Visium HD data analysis and third-party tools
Data analysis
Space Ranger Analysis Pipelines
Free analysis pipelines that process the input file types to align Visium sequencing data with the tissue image and perform optional cell segmentation.
Loupe Browser
Desktop application for Windows and MacOS that allows you to interactively visualize data.
Cloud Analysis
Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser.
Featured datasets
Related products
Visium HD 3’
Species agnostic 3’ poly(A) capture-based spatial gene expression assay for expanded whole transcriptome discovery applications.
Visium v2 WT Panel
Probe-based spatial gene expression assay utilizing first-generation slide architecture with a large Capture Area option.
Visium v1 3’
3’ poly(A) capture-based spatial gene expression assay utilizing first-generation slide architecture. Upgrading to HD 3’ is recommended.