Uncover the ‘Where’ for every ‘What’
The relationship between cells and their relative locations within a tissue sample can be critical to understanding disease pathology. Spatial transcriptomics is a groundbreaking technology that allows scientists to measure all the gene activity in a tissue sample and map where the activity is occurring. Already this technology is leading to new discoveries that will prove instrumental in helping scientists gain a better understanding of biological processes and disease.
Explore Gene Expression in the Context of the Tissue Microenvironment
Innovation in spatial gene expression technologies is enabling scientists to get a holistic understanding of cells in their morphological context. In this presentation, you will hear first-hand from 10x Genomics’ scientists about ground-breaking improvements to the technology and exciting applications showcased by users.
Gain a holistic understanding of gene and protein expression in the tissue microenvironment
Multimodal Analysis of Composition and Spatial Architecture in Human Squamous Cell Carcinoma
Multimodal Analysis of Composition and Spatial Architecture in Human Squamous Cell Carcinoma
Cell, 2020, AL Ji et al.
Cell, 2020, AL Ji et al.
Spatial Transcriptomics and In Situ Sequencing to Study Alzheimer's Disease
Spatial Transcriptomics and In Situ Sequencing to Study Alzheimer's Disease
Cell, 2020, WT Chen et al.
Cell, 2020, WT Chen et al.
Visualization and Analysis of Gene Expression in Tissue Sections By Spatial Transcriptomics
Visualization and Analysis of Gene Expression in Tissue Sections By Spatial Transcriptomics
Science. 2016, Ståhl PL et al.
Science. 2016, Ståhl PL et al.
Technology Highlight
Beyond gene expression
Turnkey Solution
Standard Tools
Streamlined data analysis
How It Works
A fresh-frozen tissue section is imaged for histological purposes (either H&E for morphological context or immunofluorescence for protein co-detection) and placed on an array containing capture probes that bind to RNA. Tissue is fixed and permeabilized to release RNA to bind to adjacent capture probes, allowing for the capture of gene expression information. cDNA is then synthesized from captured RNA and sequencing libraries prepared. The libraries are then sequenced and data visualized to determine which genes are expressed, and where, as well as in what quantity.
