Gene Expression. Visualized.
Uncover the ‘Where’ for
every ‘What’ with Spatial
The relationship between cells and their relative locations within a tissue sample can be critical to understanding disease pathology. Spatial transcriptomics is a groundbreaking technology that allows scientists to measure all the gene activity in a tissue sample and map where the activity is occurring. Already this technology is leading to new discoveries that will prove instrumental in helping scientists gain a better understanding of biological processes and disease.
Explore Gene Expression in the Context of the Tissue Microenvironment
Innovation in spatial gene expression technologies is enabling scientists to get a holistic understanding of cells in their morphological context. In this presentation, you will hear first-hand from 10x Genomics’ scientists about ground-breaking improvements to the technology and exciting applications showcased by users.
How it Works
A fresh-frozen tissue section is imaged for histological purposes and placed on an array containing capture probes that bind to RNA. Tissue is fixed and permeabilized to release RNA to bind to adjacent capture probes, allowing for the capture of gene expression information. cDNA is then synthesized from captured RNA and sequencing libraries prepared. The libraries are then sequenced and data visualized to determine which genes are expressed, and where, as well as in what quantity.
The data generated by Spatial Transcriptomics technology allows you to choose any gene of interest and display its spatially resolved expression on the original tissue section. Since all mRNAs are captured, you are not limited to visualizing only a single gene but can choose any number of genes in any combination to view and analyze together.
Use the sliders under the tissue image to adjust how you visualize and combine the tissue image and the gene expression data. Colors represent clusters identified by differentially expressed genes.
Change map spot opacity:
Change map cluster cloud opacity:
VISIUM SPATIAL GENE EXPRESSION SOLUTION
For any application involving sectioning tissue – morphological and proteomic information are incredibly important in resolving biology. Layer unbiased gene expression data onto your images and access a new level of information. This complement to your stain of interest will help you understand tissue microenvironments like never before.
Most traditional sectioning techniques and samples are supported with fresh-frozen tissues. Follow 10x Visium’s easy to follow protocols for a shorter workflow that takes your sample through to sequence-ready libraries.
Sectioning, H&E, and imaging can all be done with your own existing infrastructure. We supply the array slides, assay,and instructions for sequencing and analysis. We make it easy for you to operationalize tools in your current infrastructure.
Easy, Informative Data Analysis
Our Cell Ranger tool allows you to analyze Visium’s data outputs in the context of the tissue H&E image. We provide you the flexibility of how you analyze your data with a simple user interface
Gain a holistic understanding of gene expression
in the tissue microenvironment
See how Spatial Transcriptomics technology is being used by researchers to elevate the understanding of complex transcriptional landscapes within a spatial context, which is essential for the understanding of biology and complex disease.