This document outlines four different protocols to thaw cryopreserved cell suspensions for use with 10x Genomics Single Cell assays. One or more protocols may be applicable for a specific sample type and the most appropriate protocol should be used for thawing single cell suspensions for use with 10x Genomics Single Cell assays. This document also provides guidelines in identifying the appropriate protocol(s) by providing a comparison of the protocol features, and indicating the key differences among the four thawing protocols. The protocols described in this document are being used by 10x Genomics. Other thawing methods may also be optimized to be compatible with 10x Genomics Single Cell assays.