Cell Ranger v7.2 (and later) does not support Targeted Gene Expression analysis. Use Cell Ranger v7.1 to analyze your Targeted Gene Expression libraries.
Cell Ranger v7.2 is the last version to support the analysis of Low Throughput (LT) libraries.
- Cell Ranger provides a set of analysis pipelines that process Chromium Single Cell Gene Expression data to align reads, generate Feature Barcode matrices, perform clustering and other secondary analysis, and more.
- Start by choosing a computing option to set up and run Cell Ranger.
- Cell Ranger primary analysis pipelines require FASTQ files. If needed, convert raw sequence BCL files to FASTQ format with cellranger mkfastq (alternatively, use bcl2fastq or BCL Convert from Illumina).
- Conduct primary analysis:
- The table below shows the current recommendations for which pipeline to use for each library combination. Click on the library or combination of library types to go to the appropriate documentation.
- Depending on the pipeline, go to these pages for help specifying FASTQ files for
cellranger countorcellranger multi.
For assistance with a specific library combination not documented here, please contact [email protected]
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Conduct secondary analysis to:
- Aggregate datasets with cellranger aggr.
- Reanalyze data using tunable parameters with cellranger reanalyze (currently not supported for Feature Barcode datasets).
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Find guidance and tips for troubleshooting failed Cell Ranger runs.