There are several ways to use the 10x Genomics Cloud Analysis platform for your data analysis: 1) web browser app, 2) command line (CLI) interface, and 3) prompt-based interface.
To help you decide the best method for your use case, review the pipeline analyses that are currently supported for each method in the tables below:
| Single Cell Pipeline / Analysis | 10x Cloud Analysis web browser app | 10x Cloud Analysis Command Line (CLI) interface | 10x Cloud prompt-based interface |
|---|---|---|---|
Cell Ranger count(documentation) | |||
Cell Ranger multi(documentation for sample multiplexing, Flex, and V(D)J data) | |||
Cell Ranger aggr(documentation) | |||
Cell Ranger vdj(documentation) | |||
Cell Ranger reanalyze(documentation) | |||
| Data normalization with SCTransform (documentation) | |||
| Batch correction with Harmony (documentation) | |||
| Automated cell type annotation (documentation) | |||
Cell Ranger ARC count(documentation) | |||
Cell Ranger ARC aggr(documentation ) | |||
Cell Ranger ARC reanalyze(documentation) |
| Spatial Pipeline / Analysis | 10x Cloud Analysis web browser app | 10x Cloud Analysis Command Line (CLI) interface | 10x Cloud prompt-based interface |
|---|---|---|---|
Space Ranger count(documentation) |
The supported pipeline version(s) and library types are listed by 10x Cloud Analysis method in the tables below.
Refer to the web browser app documentation for usage details.
| Analysis Pipelines | Pipeline Version | Library Types |
|---|---|---|
Space Ranger count |
|
|
Cell Ranger count |
|
|
Cell Ranger multi |
|
|
Cell Ranger aggr |
|
|
Cell Ranger vdj1 |
|
|
Cell Ranger reanalyze2 |
|
|
| SCTransform/Harmony data integration workflow | 1.0 |
|
| Cell Ranger annotate (Beta) | 0.2 |
|
Cell Ranger ARC count | 2.1, 2.0 |
|
Cell Ranger ARC aggr | 2.1, 2.0 |
|
Cell Ranger ARC reanalyze2 | 2.1, 2.0 |
|
1 The 10x Cloud Platform does not support Cell Ranger vdj in de novo mode.
2Reanalysis of aggregated analyses (Cell Ranger) is not available in Cloud Analysis for the web browser.
Refer to the command-line interface documentation for installation and usage details.
| Analysis Pipelines | Pipeline Version | Library Types |
|---|---|---|
Space Ranger count | 4.0 |
|
Cell Ranger count | 9.0 |
|
Cell Ranger multi | 9.0 |
|
Cell Ranger aggr | 9.0 |
|
Cell Ranger vdj1 | 9.0 |
|
Cell Ranger ARC count | 2.1 |
|
Cell Ranger ARC aggr | 2.1 |
|
1 The 10x Cloud Platform does not support Cell Ranger vdj in de novo mode.
Refer to the prompt-based interface documentation for installation and usage details.
| Analysis Pipelines | Pipeline Version | Library Types |
|---|---|---|
Cell Ranger count | 9.0 |
|
Cell Ranger multi | 9.0 |
|
Cell Ranger aggr | 9.0 |
|