细胞回收率高达80%
相比之前的Next GEM技术,灵敏度提升高达100%。
更多可用的读数,测序成本节省高达50%
为什么选择通用型5’基因表达
需要全长转录本读数
通量灵活,分析每个样本中500个至2万个细胞
广泛的多组学选项,包括蛋白质、CRISPR、基因表达、TCR/BCR
分析规格
小鼠
人类
大鼠(TCR/BCR除外)
非人灵长类动物(TCR/BCR除外)
等
物种
细胞悬液
细胞核悬液
新鲜组织
冷冻组织
流式分选细胞
固定的人类PBMC
类器官
兼容样本
5’基因表达
细胞表面蛋白
TCR/BCR测序
CRISPR筛选
抗原特异性
多组学
20000个细胞/样本
160000个细胞/芯片p
8个样本/芯片
单重分析通量选项
5000个细胞/样本
40000个细胞/芯片
每个通道输出最多4个样本,每个芯片8个样本
芯片上多重分析通量选项(新!)
组件
Consumables
Nuclei Isolation (optional)
1000494
Chromium Nuclei Isolation with RNase Inhibitor Kit, 16rxns
Chip
1000698
Chromium GEM-X Single Cell 5' Chip Kit v3, 4 chips
Index Kit
1000215
Dual Index Kit TT Set A 96 rxns
1000250
Dual Index Kit TN Set A, 96 rxn
Core Reagents
1000695
Chromium GEM-X Single Cell 5' Kit v3, 4 rxns
1000699
Chromium GEM-X Single Cell 5' Kit v3, 16 rxns
1000712
Chromium GEM-X Single Cell 5' Kit v3, 48 rxns
Core Reagents (optional)
1000826
GEM-X Universal 5' Gene Expression cDNA Kit v3, 16 samples
Library Construction Kit (optional)
1000694
Library Construction Kit C, 16 rxns
1000774
Library Construction Kit C, Automated 24 rxns / Manual 32 rxns
V(D)J Amplification Kit (optional)
1000252
Chromium Single Cell Human TCR Amplification Kit, 16 rxns
1000253
Chromium Single Cell Human BCR Amplification Kit, 16 rxns
1000254
Chromium Single Cell Mouse TCR Amplification Kit, 16 rxns
1000255
Chromium Single Cell Mouse BCR Amplification Kit, 16 rxns
CRISPR Kit (optional)
1000451
5' CRISPR Kit, 16 rxns
Feature Barcoding Kit (optional)
1000703
Chromium GEM-X Single Cell 5 Feature Barcode Kit v3, 16 rxns

文档和资源
产品说明
用户指南
视频
数据分析
Cell Ranger
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data
Loupe Browser
Loupe Browser is a desktop application for Windows and MacOS that allows you to interactively visualize data
Cloud Analysis
Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser
精选文献
文献
Nature Medicine 2023 Jun Tian, et al.
Nature Cancer, 2023 Francesco Maura, et al.
Nature Medicine, 2023 Nathaniel Anderson, et al.