Resources:
多组学细胞术
高灵敏度地检测5'mRNA以及抗原特异性和表型。
完整的免疫组库
配对的全长Ig序列（包括同种型）和T细胞受体α/β链。
免疫映射
以单细胞分辨率将免疫组库和抗原特异性相结合。
简化的数据分析
使用易用的软件来探索和查看克隆型信息。
为期一天的实验室流程
在一天之内完成从样本到可测序文库的过程。
兼容不同的样本
经验证适用于PBMC、新鲜和冻存细胞样本，以及解离的组织。
探索您的应用
发现新的细胞类型和功能
全面研究免疫组库
探索抗原特异性
发现新的生物标志物
Flexible solutions to meet your needs
Single cell resolution for comprehensive immune profiling
Multiomic analysis
Simultaneously measure cell surface protein expression and antigen specificity with 5’ gene expression.
Immune Repertoire Profiling
Understand clonal expansion and diversity by capturing full-length, paired B-cell and T-cell receptors at a single cell level.
- Paired receptor sequence enables re-expression and testing for antigen binding and function
- Combined with gene expression data, perform immune cell classification and discover new cell states
Cell Surface Protein
Correlate gene expression measurements with immunophenotyping at a single cell level to better delineate immune cell subsets and functional states.
- Enable ultra-high parameter discovery, using multiomic cytometry to detect up to hundreds of proteins simultaneously with unbiased gene expression profiling
- Stratify immune cell populations by correlating protein isoforms with regulatory network co-expression in single cells
Antigen Specificity
Assess the specificity of the adaptive immune system at single cell resolution with simultaneous capture of paired T-cell receptors and peptide–MHC multimers, or immunoglobulin heavy and light chains and their cognate antigens.
- Explore public and private T-cell antigen specificities using barcoded peptide–MHC multimers
- Characterize how neoantigens may be targeted by the adaptive immune system
- Accelerate antibody discovery with a faster and higher throughput screening technology
Large-scale projects
Tackle your most ambitious projects, from rare cell type discovery to translational studies, with our high-throughput (HT) kit and complementary solutions for larger-scale projects.
Single Cell Immune Profiling HT
Transform single cell immune profiling with high-throughput (HT) multiomic analysis on the new Chromium X, and reveal the incredible diversity of the adaptive immune system.
Accelerate time to resultsAnalyze hundreds of thousands of single cells in a single chip for up to 16 samples at once.
- Enjoy economy of scale
Take advantage of high-throughput capabilities and reduce experimental and analysis costs even further with Targeted Gene Expression.
- Support from prep to analysis
From technical resources to user-friendly, cloud-based data analytics, you are supported end-to-end.
Targeted Gene Expression
Profile a defined set of transcripts to reduce costs while increasing sample number or sequencing depth with customizable, comprehensive gene panels.
- Built to scale
Maximize sample throughput and reduce hands-on time by multiplexing libraries during target enrichment.
- Seamless integration
Don’t miss any data—seamlessly transition between whole transcriptome and targeted analysis from the same library.
- More for less
As studies scale, maximize your budget by decreasing sequencing costs with pre-designed or custom targeted panels.
Workflow automation
Maximize lab productivity and obtain consistent, reproducible results with Single Cell Immune Profiling for Chromium Connect.
Automated Single Cell Gene Expression
Automate your immune profiling workflow on the Chromium Connect to explore T- and B-cell repertoire diversity, identify rare cell populations, and reveal clonal expansion in response to disease and therapy with increased productivity.
- Optimize productivity
Shorten your hands-on time, and go from single cells to sequencing-ready immune profiling libraries with walk-away convenience.
- Standardize your workflow
Generate consistent and reproducible single cell immune profiling results across experiments, users, and even across multiple sites.
- Streamline operations
Utilize pre-aliquoted, automation-specific reagents that are color-coded and 2D-barcoded for error-proof reagent tracking and loading.
经过验证的结果
经过验证的结果
文献
搜索百余篇采用Chromium单细胞免疫分析的同行评审文献
High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity
High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity
Cell. 2019, Ian Setliff, et al.
Cell. 2019, Ian Setliff, et al.
Massively Parallel Single-Cell B-Cell Receptor Sequencing Enables Rapid Discovery of Diverse Antigen-Reactive Antibodies
Massively Parallel Single-Cell B-Cell Receptor Sequencing Enables Rapid Discovery of Diverse Antigen-Reactive Antibodies
Communications Biology. 2019, Leonard D. Goldstein, et al.
Communications Biology. 2019, Leonard D. Goldstein, et al.
Clonal replacement of tumor-specific T cells following PD-1 blockade
Clonal replacement of tumor-specific T cells following PD-1 blockade
Nature Medicine. 2019, Katheryn E. Yost, et al.
Nature Medicine. 2019, Katheryn E. Yost, et al.
A cell atlas of human thymic development defines T cell repertoire formation
A cell atlas of human thymic development defines T cell repertoire formation
Science. 2020, Jong-Eun Park, et al.
Science. 2020, Jong-Eun Park, et al.
资源
查找有关Chromium单细胞免疫分析的最新应用指南及其他文档
A New Way of Exploring Immunity—Linking Highly Multiplexed Antigen Recognition to Immune Repertoire and Phenotype
A New Way of Exploring Immunity—Linking Highly Multiplexed Antigen Recognition to Immune Repertoire and Phenotype
Application Note, 10x Genomics
Application Note, 10x Genomics
Improving Single Cell Characterization with Simultaneous Gene Expression and Cell Surface Protein Measurements at Scale
Improving Single Cell Characterization with Simultaneous Gene Expression and Cell Surface Protein Measurements at Scale
Application Note, 10x Genomics
Application Note, 10x Genomics
Characterization of Tumor Microenvironment
Characterization of Tumor Microenvironment
Application Note, 10x Genomics
Application Note, 10x Genomics
完整的解决方案
Chromium单细胞免疫分析试剂
利用我们的试剂盒，您可以从多个维度探索适应性免疫力，包括5’ mRNA、配对的全长BCR和TCR序列、抗原-受体特异性、细胞表面蛋白等。
世界级的技术支持和客户支持
您可以通过电话或电子邮件联系我们的专家支持团队。
工作流程
单细胞免疫分析的基本流程。若您使用我们的Feature Barcoding试剂盒来分析细胞表面蛋白或抗原特异性，我们将会提供其他方案用于修饰您感兴趣的蛋白。
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文库构建
利用我们的试剂盒和Chromium Controller构建带有10x条形码的文库。Chromium Controller将每个带有10x条形码的凝胶珠的细胞封装在单个液滴中。在每个纳升级的液滴中，细胞经过逆转录生成cDNA，其中单个细胞的所有cDNA都带有共同的10x条形码。
资源 - 3
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数据分析
免费下载的Cell Ranger可将原始的测序数据转化成有生物学意义的见解。Cell Ranger可处理重链/轻链测序、基因表达和抗原特异性的数据。
分析流程输出
输出结果包括质控信息和文件，这些文件可轻松用于Loupe可视化软件或第三方R工具及Python工具的进一步分析。
资源 - 5
数据可视化
利用我们的Loupe Browser可视化软件，您可以交互式地探索实验结果，并比较不同样本和细胞类型之内或之间的详细克隆型信息，以研究基因使用、组库多样性、克隆扩增等。
只有生物信息学家才能使用吗？
Loupe是点选式软件，任何人都可以轻松下载和使用。
资源
常见问题
该解决方案能够对来自单个细胞的多个类型的数据进行模块化整合，包括基于mRNA的全转录组或靶向基因表达、免疫分型、适应性免疫组库的克隆性和多样性，以及抗原特异性。这种多组学的细胞鉴定方法有助于通过一次实验破译单个细胞的免疫系统规则。
可以。您可以只扩增免疫球蛋白或TCR文库用于测序，而将扩增好的全转录组cDNA样本进行保留，用于后续测序。
Chromium单细胞免疫分析与新鲜或冻存的单细胞悬液兼容。典型的样本包括PBMC、肿瘤浸润淋巴细胞、脾脏、骨髓细胞及其他组织类型。兼容的物种包括人、小鼠、大鼠及其他模式生物。不过，10x Genomics的免疫球蛋白或TCR测序试剂和方案仅支持人和小鼠。
Chromium单细胞免疫分析解决方案利用针对Ig和TCR基因恒定区的基因特异性扩增引物来富集和测序整个V(D)J基因片段。
可以，免疫球蛋白的重链序列可分为IgA、IgD、IgE、IgM和IgG。
