Chromium单细胞分析

Universal 5' Gene Expression

GEM-X technology-powered assays with whole transcriptome coverage to analyze immune diversity

细胞回收率高达80%

相比之前的Next GEM技术,灵敏度提升高达100%。

更多可用的读数,测序成本节省高达50%

为什么选择通用型5’基因表达

  • 需要全长转录本读数

  • 通量灵活,分析每个样本中500个至2万个细胞

  • 广泛的多组学选项,包括蛋白质、CRISPR、基因表达、TCR/BCR

分析规格

    物种

  • 小鼠

  • 人类

  • 大鼠(TCR/BCR除外)

  • 非人灵长类动物(TCR/BCR除外)

    兼容样本

  • 细胞悬液

  • 细胞核悬液

  • 新鲜组织

  • 冷冻组织

  • 流式分选细胞

  • 固定的人类PBMC

  • 类器官

    多组学

  • 5’基因表达

  • 细胞表面蛋白

  • TCR/BCR测序

  • CRISPR筛选

  • 抗原特异性

    单重分析通量选项

  • 20000个细胞/样本

  • 160000个细胞/芯片p

  • 8个样本/芯片

    芯片上多重分析通量选项(新!)

  • 5000个细胞/样本

  • 40000个细胞/芯片

  • 每个通道输出最多4个样本,每个芯片8个样本

  • 了解更多

组件

每个实验都需要芯片、索引试剂盒、核心试剂以及可选的feature barcoding试剂盒。如果您不确定自己需要什么,请使用实验构建工具查找实验所需的自定义组分列表。

Consumables


Nuclei Isolation (optional)

REAGENT

1000494

Chromium Nuclei Isolation with RNase Inhibitor Kit, 16rxns

Chip

CHIP

1000698

Chromium GEM-X Single Cell 5' Chip Kit v3, 4 chips

Index Kit

REAGENT

1000215

Dual Index Kit TT Set A 96 rxns

REAGENT

1000250

Dual Index Kit TN Set A, 96 rxn

Core Reagents

REAGENT

1000695

Chromium GEM-X Single Cell 5' Kit v3, 4 rxns

REAGENT

1000699

Chromium GEM-X Single Cell 5' Kit v3, 16 rxns

REAGENT

1000712

Chromium GEM-X Single Cell 5' Kit v3, 48 rxns

Core Reagents (optional)

REAGENT

1000826

GEM-X Universal 5' Gene Expression cDNA Kit v3, 16 samples

Library Construction Kit (optional)

REAGENT

1000694

Library Construction Kit C, 16 rxns

REAGENT

1000774

Library Construction Kit C, Automated 24 rxns / Manual 32 rxns

V(D)J Amplification Kit (optional)

REAGENT

1000252

Chromium Single Cell Human TCR Amplification Kit, 16 rxns

REAGENT

1000253

Chromium Single Cell Human BCR Amplification Kit, 16 rxns

REAGENT

1000254

Chromium Single Cell Mouse TCR Amplification Kit, 16 rxns

REAGENT

1000255

Chromium Single Cell Mouse BCR Amplification Kit, 16 rxns

CRISPR Kit (optional)

REAGENT

1000451

5' CRISPR Kit, 16 rxns

Feature Barcoding Kit (optional)

REAGENT

1000703

Chromium GEM-X Single Cell 5 Feature Barcode Kit v3, 16 rxns

Compatible Instruments


Chromium XChromium iX

构建属于您的通用型5‘基因表达实验

设计适合您的实验

视频

数据分析

Cell Ranger image

Cell Ranger

Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data

Loupe Browser image

Loupe Browser

Loupe Browser is a desktop application for Windows and MacOS that allows you to interactively visualize data

Cloud Analysis image

Cloud Analysis

Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser

精选数据集

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Single cell profiling of open chromatin and 3' gene expression from the same nucleus

Epi ATAC

Single cell profiling of open chromatin

Chromium Single Cell

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