Universal 5'
Up to 80% cell recovery
Up to 100% higher sensitivity vs prior Next GEM tech
More usable reads, save up to 50% on sequencing costs
Why choose Universal 5' Gene Expression?
Require full-length transcript readouts
Flexible throughput from 500 to 20K cells per sample
Broad multiomic options including protein, CRISPR, gene expression, TCR/BCR, and antigen specificity
Assay specifications
Mouse
Human
Rat (except with TCR/BCR)
Non-human primate (except with TCR/BCR)
and more
Species
Cell suspension
Nuclei suspension
Fresh tissue
Frozen tissue
Flow-sorted cells
Fixed human PBMCs
Organoids
Compatible samples
5' gene expression
Cell surface protein
TCR/BCR sequencing
CRISPR screening
Antigen specificity
Multiomics
Compatible instruments
20K cells/sample
160K cells/chip
8 samples/chip
Singleplex throughput options
5K cells/sample
40K cells/chip
Up to 4 samples/channel output, 8 samples/chip
On-chip multiplexing throughput options (New!)
Components
Consumables
Choose a version
Nuclei Isolation (optional)
1000494
Chromium Nuclei Isolation with RNase Inhibitor Kit, 16rxns
Chip
1000748
Kit, GEM-X OCM 5' Chip Kit v3 4-plex, 2 chips
1000955
Kit, GEM-X OCM 5' Chip Kit v3 4-plex, 1 chip
Index Kit
1000215
Dual Index Kit TT Set A 96 rxns
1000242
Dual Index Kit NT Set A, 96 rxn
Core Reagents
1000780
GEM-X Universal 5’ Gene Expression v3 4-plex, 16 samples
1000956
GEM-X Universal 5’ Gene Expression v3 4-plex, 8 samples
Core Reagents (optional)
1000826
GEM-X Universal 5' Gene Expression cDNA Kit v3, 16 samples
Library Construction Kit (optional)
1000694
Library Construction Kit C, 16 rxns
1000774
Library Construction Kit C, Automated 24 rxns / Manual 32 rxns
V(D)J Amplification Kit (optional)
1000252
Chromium Single Cell Human TCR Amplification Kit, 16 rxns
1000253
Chromium Single Cell Human BCR Amplification Kit, 16 rxns
1000254
Chromium Single Cell Mouse TCR Amplification Kit, 16 rxns
1000255
Chromium Single Cell Mouse BCR Amplification Kit, 16 rxns
CRISPR Kit (optional)
1000451
5' CRISPR Kit, 16 rxns
Feature Barcoding Kit (optional)
1000703
Chromium GEM-X Single Cell 5 Feature Barcode Kit v3, 16 rxns
Build your own Universal 5' Gene Expression experiment
Design the experiment that's right for you
Documents and resources
Videos
Data analysis
Cell Ranger
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data
Loupe Browser
Desktop application for Windows and MacOS that allows you to interactively visualize data.
Cloud Analysis
Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser
Featured publications
Publications
Nature Medicine 2023 Jun Tian, et al.
Nature Cancer, 2023 Francesco Maura, et al.
Nature Medicine, 2023 Nathaniel Anderson, et al.
Featured datasets
Related products
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Combined profiling of 3' gene expression and chromatin accessibility from the same cell.
