Chromium Nuclei Isolation

Remove the complexities of sample preparation with a streamlined workflow

Easily and efficiently prepare frozen samples for single nuclei sequencing, consolidating your experimental processes and improving access to more samples. Ensure reliable results from multiple tissue types with a unified sample prep workflow optimized for use with Chromium gene expression and chromatin accessibility assays.

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Nuclei Isolation

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  • Get started today

    Easy to use, no single cell sequencing expertise required.

  • Save time and precious samples

    Take the guesswork out of optimizing tissue dissociation and nuclei isolation.

  • Be confident in your results

    Reliable, standardized reagents designed for 10x Genomics single cell assays.

Resolve true tissue composition

Characterize the heterogeneity of tissues more completely by preserving cells often lost during fresh tissue dissociation. Leverage streamlined nuclei isolation to avoid long enzymatic incubations that can alter gene expression and create cell-type biases.

Reveal gene regulation mechanisms

Use nuclei to capture gene expression and chromatin accessibility information to explore gene regulatory interactions driving cell differentiation, development, and disease.

Preserve the biological state of your sample

Freeze your sample in time and preserve the biological state. Transport your samples for sample preparation at a central facility or store them for a later date, minimizing changes in gene expression.

Expand your reach to stored samples

Reevaluate biobanked frozen tissues using new single cell technologies, and address scientific questions historically left unanswered.

Proven Results

Watch how customers are using the Chromium Single Cell Nuclei Isolation Kit.

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Find the latest app notes and other documentation on the Chromium Single Cell Nuclei Isolation Kit.

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Proven Results

Videos

Watch how customers are using the Chromium Single Cell Nuclei Isolation Kit.

View all

Resources

Find the latest app notes and other documentation on the Chromium Single Cell Nuclei Isolation Kit.

Sample preparation for single cell applications in oncology

Sample preparation for single cell applications in oncology

Sample Preparation Guide, 10x Genomics

Sample Preparation Guide, 10x Genomics

Sample preparation optimization guide with Single Cell Gene Expression LT

Sample preparation optimization guide with Single Cell Gene Expression LT

Sample Preparation Guide, 10x Genomics

Sample Preparation Guide, 10x Genomics

Rapid, scalable isolation of human tumor nuclei for single cell genomics

Rapid, scalable isolation of human tumor nuclei for single cell genomics

Poster, AACR

Poster, AACR

Our end-to-end solution

Nuclei preparation

All-in-one kit uses standard laboratory equipment, no special instrumentation needed.

Select your single cell assay

Nuclei can be used to capture chromatin accessibility or whole transcriptome gene expression profiles.

Analysis and visualization software

Our analysis pipelines

Our visualization software

World-class technical and customer support

Our expert support team can be contacted by phone or email.

Workflow

Scroll down to see the base workflow for Chromium Nuclei Isolation.

  1. 1Step 1

    Obtain your sample

    Start with your frozen tissue of interest. Compatible sample types include mouse heart, liver, brain, and a variety of human tumors.

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  2. 2Step 2

    Break apart your frozen sample

    Use the provided pestle and lysis buffer to mechanically disrupt the frozen tissue into a homogeneous mix.

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  3. 3Step 3

    Isolate nuclei

    Pass the homogenized tissue through the provided spin column to isolate nuclei from the disrupted sample.

  4. 4Step 4

    Remove debris

    Clean up the nuclei suspension using the supplied debris removal solution and buffer washes.

  5. 5Step 5

    Resuspend nuclei and QC

    Resuspend the nuclei in the recommended resuspension buffer, check nuclei quality, and proceed to your single cell assay of choice.

    Resources

Frequently asked questions

The Chromium Nuclei Isolation Kit has been validated with the following frozen tissues: mouse heart, liver, brain, lung, kidney, small intestine, and a variety of human tumors. An up-to-date list of tissues tested is available on the 10x Genomics Support site. This kit is not compatible with cell suspensions, plant or insect material, calcified tissues, or FFPE tissues.

Supported tissue sizes range from 3-50 mg per column. For pieces of tissue which are larger, break up the tissue into 50 mg pieces, run multiple dissociation columns, and combine nuclei at the final step. It may be possible to run pieces <3 mg, but yield is dependent on tissue type.

All the consumables and reagents needed to generate a clean nuclei suspension are included in the kit and utilize standard lab equipment. No specialized instrument is needed.

Compatible single cell assays include Chromium Single Cell ATAC, Single Cell Multiome ATAC + Gene Expression, Single Cell Gene Expression, and Single Cell Gene Expression Flex. Follow the nuclei resuspension recommendations for the specific assay you are running. No modifications to the single cell workflows are necessary.

10x Genomics provides analysis and visualization software to help you analyze your single cell data. Follow analysis recommendations for the single cell assay of interest. For single cell gene expression assays, make sure introns are included during analysis. You can download Cell Ranger and Loupe Browser software from the 10x Genomics Support site at no cost.

Single Cell Gene Expression

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