Chromium Single Cell

Universal 3'

GEM-X technology-powered assays with whole transcriptome coverage to gather broad information from diverse species

Up to 80% cell recovery

Up to 100% higher sensitivity vs prior Next GEM tech

More usable reads, save up to 50% on sequencing costs

Why choose Universal 3' Gene Expression?

  • Require full-length transcript readouts

  • Flexible throughput from 500 to 20K cells per sample

  • Broad multiomic options including protein and gene expression

Assay specifications

    Species

  • Human

  • Mouse

  • Rat

  • Non-human primate

  • Marine

  • Plant

  • Insect

  • and more

    Compatible samples

  • Cell suspension

  • Nuclei suspension

  • Fresh tissue

  • Frozen tissue

  • Flow-sorted cells

  • Fixed human PBMCs

  • Organoids

    Multiomics

  • 3' gene expression

  • Cell surface protein

    Singleplex throughput options

  • 20K cells/sample

  • 160K cells/chip

  • 8 samples/chip

Components

Each experiment requires a chip, index kit, and core reagents plus an optional feature barcoding kit. If you're unsure what you need, use the experiment builder to find the custom list of items required for your experiment.

Consumables

Choose a version


Nuclei Isolation (optional)

REAGENT

1000494

Chromium Nuclei Isolation with RNase Inhibitor Kit, 16rxns

Chip

CHIP

1000747

Kit, GEM-X OCM 3' Chip Kit v4 4-plex, 2 chips

CHIP

1000953

Kit, GEM-X OCM 3' Chip Kit v4 4-plex, 1 chip

Index Kit

REAGENT

1000215

Dual Index Kit TT Set A 96 rxns

REAGENT

1000242

Dual Index Kit NT Set A, 96 rxn

Core Reagents

REAGENT

1000779

GEM-X Universal 3’ Gene Expression v4 4-plex, 16 samples

REAGENT

1000957

GEM-X Universal 3’ Gene Expression v4 4-plex, 8 samples

Core Reagents (optional)

REAGENT

1000825

GEM-X Universal 3' Gene Expression cDNA Kit v4, 16 samples

Library Construction Kit (optional)

REAGENT

1000694

Library Construction Kit C, 16 rxns

REAGENT

1000774

Library Construction Kit C, Automated 24 rxns / Manual 32 rxns

Feature Barcoding Kit (optional)

REAGENT

1000702

Chromium GEM-X Single Cell 3 Feature Barcode Kit v4, 16 rxns

RNase Inhibitor (Optional)

REAGENT

1000887

Kit, RNase Inhibitor 40x

Compatible Instruments


Chromium XChromium Xo

Build your own Universal 3' Gene Expression experiment

Design the experiment that's right for you

Documents and resources

Videos

Cell Ranger image

Cell Ranger

Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data.

Loupe Browser image

Loupe Browser

Desktop application for Windows and MacOS that allows you to interactively visualize data.

Cloud Analysis image

Cloud Analysis

Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser.

Featured publications

Featured datasets

Flex

Fixed RNA Profiling assay for comprehensive probe-based gene expression profiling with single cell resolution.

Universal 5'

5' gene expression alongside V(D)J repertoire profiling and antigen specificity of T and B cells.

Epi Multiome ATAC + Gene Expression

Combined profiling of 3' gene expression and chromatin accessibility from the same cell.

Epi ATAC

Chromatin accessibilility profiling at the single cell level.

Chromium Single Cell

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