Visium Spatial Proteogenomics

Whole tissue profiling of RNA and protein from a single section

Combine whole transcriptome spatial analysis with highly multiplexed protein detection in the same human FFPE tissue section. Gain a deeper, more holistic understanding of tissue organization with simultaneous gene and protein expression analysis.

Spatial Proteogenomics

Resources:

  • Perform single-section proteogenomics

    Perform single-section proteogenomics

    Get deeper insights into human FFPE tissue with simultaneous protein, whole transcriptome, and morphology data from a single section.

  • Get started with our validated antibody panel

    Get started with our validated antibody panel

    Obtain greater insights with a predesigned, 35-plex human immune cell profiling antibody panel.

  • Retain morphological context

    Retain morphological context

    Compatible with hematoxylin and eosin (H&E) and immunofluorescence (IF) stains.

  • Profile entire tissue sections

    Profile entire tissue sections

    Visualize protein and whole transcriptome expression profiles across an entire human FFPE tissue section.

  • See in high resolution

    See in high resolution

    Capture a clearer picture of your tissue with an average resolution of 1–10 cells per spot depending on tissue type.

  • Streamline data analysis

    Streamline data analysis

    Combine gene and protein expression data with a histological image with our easy-to-use software.

What you can do

Map the spatial organization of cell atlases

Add more dimensions to cell atlases by combining whole transcriptome, high-plex protein, and morphological analyses on the same tissue section

Obtain in-depth characterization of immune cells

Pair immune cell marker proteins with gene expression to deeply understand the location and heterogeneity of immune cells within the tumor microenvironment

Use protein to select key regions of interest

Guide your whole transcriptome analysis by honing in on biologically significant tissue regions with over 30 immune cell protein targets

Proven results

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Proven results

Videos

Watch how customers are using Spatial Gene Expression.

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Resources

Find the latest app notes and other documentation Spatial Gene Expression.

Inside Visium spatial capture technology

Inside Visium spatial capture technology

Brochure, 10x Genomics

Brochure, 10x Genomics

Resolving brain architecture with comprehensive spatial gene expression

Resolving brain architecture with comprehensive spatial gene expression

Data Spotlight, 10x Genomics

Data Spotlight, 10x Genomics

Our end-to-end solution

Visium Spatial Slides for FFPE v2

Visium Spatial Slides for FFPE v2

Easily adoptable within existing lab infrastructure, no need for a new instrument.

Visium Spatial Gene and Protein Expression reagents

Visium Spatial Gene and Protein Expression reagents

Our reagent kits let you capture whole-transcriptome gene and multiplexed protein expression with spatial resolution across an entire tissue section.

Analysis and visualization software

Analysis and visualization software

Space Ranger

Our analysis pipelines

Our visualization software

World-class technical and customer support

World-class technical and customer support

Get the help you need with our expert support team, reachable by phone or e-mail.

Workflow

  1. 1Step 1

    Prepare your sample

    Embed, section, and place human FFPE tissue onto a Capture Area of the gene expression slide. Each Capture Area has thousands of barcoded spots containing millions of capture oligonucleotides with spatial barcodes unique to that spot.

    Resources
    • FFPE How-to Videos - coming soon

    • FFPE Tissues Tested - coming soon

  2. 2Step 2

    Stain and image the tissue

    Use standard fixation and staining techniques, including H&E staining to visualize tissue sections with a brightfield imaging system, or IF staining to visualize up to three key protein markers in tissue sections with a fluorescent imaging system.

    Resources
    • FFPE Staining and Imaging Protocol - coming soon

    • FFPE User Guide - coming soon

  3. 3Step 3

    Permeabilize tissue and construct library

    The FFPE tissue is permeabilized to release ligated probe pairs and antibody tags from the cells, which bind to the spatially barcoded oligonucleotides present on the spots. Spatial barcodes are added via an extension reaction. The barcoded molecules are then pooled for downstream processing to generate a sequencing-ready library.

    Resources
    • FFPE User Guide - coming soon

  4. 4Step 4

    Sequence

    The resulting 10x Barcoded libraries are compatible with standard NGS short-read sequencing on Illumina sequencers for transcriptional and protein profiling of entire tissue sections.

    Resources
    • FFPE Sequencing Requirements - coming soon

  5. 5Step 5

    Analyze and visualize your data

    Use our Space Ranger analysis software to process your Spatial Gene and Protein Expression data, and interactively explore the results with our Loupe Browser visualization software.

    Do I need to be a bioinformatician to use Loupe?

    Loupe is a point-and-click software that’s easy for anyone to download and use.

    Resources
    • Space Ranger Analysis Pipelines
    • Loupe Visualization Software
    • Downloadable Datasets - coming soon

Frequently asked questions

The Visium Spatial Gene and Protein Expression workflow is compatible with H&E and IF from the same tissue section. For IF we support up to four protein markers and recommend that DAPI be used for one of the channels. Please review our imaging guidelines for additional information.

Imaging Guidelines - coming soon

Staining Demonstrated Protocols - coming soon

The human immune panel includes antibodies that detect cell surface (extracellular) and intracellular protein targets. The full list of antibodies in the panel can be found here.

No, the addition of protein does not affect the sensitivity of gene expression in the Visium Gene and Protein Expression assay.

The Visium Gene and Protein Expression assay is compatible with human FFPE tissues. A list of tissues successfully tested in-house with the Visium assay can be found below.

Successfully tested tissues: Coming soon

Single Cell Gene Expression

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