Visium Spatial
v1 3’ Gene Expression
First-generation Visium technology–powered assay with whole transcriptome coverage to gather broad information from diverse species.
Upgrade to Visium HD technology for single cell–scale resolution and gapless tissue coverage
Explore HD 3' Gene ExpressionFirst-generation slide architecture provides 1–10 cell resolution
Assay specifications
Species
AgnosticCompatible samples
Fresh frozen tissueMultiomics
3’ Gene expression
Protein
Morphology
Isoform expression
TCR & BCR sequencingCompatible instruments
Throughput options
Assay components
Each experiment requires Visium Spatial Gene Expression (v1) slides and core reagents. If you’re unsure what you need, use the experiment builder to find the custom list of items required for your experiment.
Consumables
Index Kit
1000215
Dual Index Kit TT Set A, 96 rxn
Core Slides and Reagents
1000187
Visium Gene Expression Slide and Reagent Kit, 4 rxns
1000184
Visium Gene Expression Slide and Reagent Kit, 16 rxns
Starter Kits
1000193
Visium Tissue Optimization Slide and Reagent Kit, 4 slides
1000200
Visium Spatial Gene Expression Starter Kit
RNAase Inhibitor (Optional)
1000887
Kit, RNase Inhibitor 40x
Documents and resources
User guides
Technical notes
Sequencing requirements
The final gene expression library is sequenced at a recommended minimum depth of 50K read pairs per capture spot covered by tissue. For some samples, fewer reads will be sufficient, while more complex samples may require more reads.
View moreData analysis
Space Ranger Analysis Pipelines
Free analysis pipelines that process the input file types to align Visium sequencing data with the tissue image and perform optional cell segmentation.
Learn about Space Ranger
Loupe Browser
Desktop application for Windows and MacOS that allows you to interactively visualize data.
Download Loupe BrowserFeatured datasets
Human Cerebellum: Whole Transcriptome Analysis
View datasetMouse Kidney Section (Coronal)
View datasetRelated products
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Probe-based spatial gene expression assay for comprehensive differential gene expression at single cell scale.
Visium HD 3’
Species agnostic 3’ poly(A) capture-based spatial gene expression assay for expanded whole transcriptome discovery applications.
Visium v2 WT Panel
Probe-based spatial gene expression assay utilizing first-generation slide architecture with a large Capture Area option.