Visium Spatial

v1 3’ Gene Expression

First-generation Visium technology–powered assay with whole transcriptome coverage to gather broad information from diverse species.

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Upgrade to Visium HD technology for single cell–scale resolution and gapless tissue coverage

Explore HD 3' Gene Expression

First-generation slide architecture provides 1–10 cell resolution

Assay specifications

Species

Agnostic

Compatible samples

Fresh frozen tissue

Multiomics

3’ Gene expression

Protein

Morphology

Isoform expression

TCR & BCR sequencing

Compatible instruments

Direct placement

Throughput options

Capture Area size: 6.5 x 6.5 mm

Assay components

Each experiment requires Visium Spatial Gene Expression (v1) slides and core reagents. If you’re unsure what you need, use the experiment builder to find the custom list of items required for your experiment.

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Consumables

Index Kit

REAGENT

1000215

Dual Index Kit TT Set A, 96 rxn

Core Slides and Reagents

REAGENT

1000187

Visium Gene Expression Slide and Reagent Kit, 4 rxns

REAGENT

1000184

Visium Gene Expression Slide and Reagent Kit, 16 rxns

Starter Kits

REAGENT

1000193

Visium Tissue Optimization Slide and Reagent Kit, 4 slides

REAGENT

1000200

Visium Spatial Gene Expression Starter Kit

RNAase Inhibitor (Optional)

REAGENT

1000887

Kit, RNase Inhibitor 40x

Sequencing requirements

The final gene expression library is sequenced at a recommended minimum depth of 50K read pairs per capture spot covered by tissue. For some samples, fewer reads will be sufficient, while more complex samples may require more reads.