Visium Spatial

v1 3' Gene Expression

First-generation Visium technology-powered assay with whole transcriptome coverage to gather broad information from diverse species.

Upgrade to Visium HD technology for single cell-scale resolution and gapless tissue coverage

First-generation slide architecture provides 1–10 cell resolution

Assay specifications

    Species

  • Agnostic

    Compatible samples

  • Fresh frozen tissue

    Multiomics

  • 3' Gene expression

  • Protein

  • Morphology

  • Isoform expression

  • TCR & BCR sequencing

    Compatible instruments

    Direct placement

    Throughput options

    Capture Area size: 6.5 x 6.5 mm

Assay components

Each experiment requires Visium Spatial Gene Expression (v1) slides and core reagents. If you're unsure what you need, use the experiment builder to find the custom list of items required for your experiment.

Consumables


Index Kit

REAGENT

1000215

Dual Index Kit TT Set A, 96 rxn

Core Slides and Reagents

REAGENT

1000187

Visium Gene Expression Slide and Reagent Kit, 4 rxns

REAGENT

1000184

Visium Gene Expression Slide and Reagent Kit, 16 rxns

Starter Kits

REAGENT

1000193

Visium Tissue Optimization Slide and Reagent Kit, 4 slides

REAGENT

1000200

Visium Spatial Gene Expression Starter Kit

RNase Inhibitor (Optional)

REAGENT

1000887

Kit, RNase Inhibitor 40x

Documents and resources

User guides

Sequencing requirements

The final gene expression library is sequenced at a recommended minimum depth of 50K read pairs per capture spot covered by tissue. For some samples, fewer reads will be sufficient, while more complex samples may require more reads.

View more
Space Ranger Analysis Pipelines image

Space Ranger Analysis Pipelines

Free analysis pipelines that process the input file types to align Visium sequencing data with the tissue image and perform optional cell segmentation.

Loupe Browser image

Loupe Browser

Desktop application for Windows and MacOS that allows you to interactively visualize data.

Featured datasets

Related products

Visium HD WT Panel

Probe-based spatial gene expression assay for comprehensive differential gene expression at single cell scale.

Visium HD 3'

Species agnostic 3' poly(A) capture-based spatial gene expression assay for expanded whole transcriptome discovery applications.

Visium v2 WT Panel

First-generation Visium technology-powered assay with protein coding gene coverage and immuno-oncology protein panel.

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