Visium Spatial
v2 WT Panel Gene Expression
First-generation Visium technology–powered assay with protein coding gene coverage and immuno-oncology protein panel.
Upgrade to Visium HD technology for single cell–scale resolution and gapless tissue coverage
Explore HD WT Panel Gene ExpressionFirst-generation slide architecture provides 1–10 cell resolution
Why choose v2 Gene Expression
Assay specifications
Species
Mouse
HumanCompatible samples
FFPE tissue
Fresh frozen tissue
Fixed frozen tissueMultiomics
Probe-based Gene expression
Protein
Morphology
Human immuno-oncology protein panelCompatible instruments
Throughput options
Assay components
Each experiment requires Visium Spatial Gene Expression (v2) slides, core reagents, and the Visium CytAssist instrument. If you’re unsure what you need, use the experiment builder to find the custom list of items required for your experiment.
Consumables
Index Kit
1000251
Dual Index Kit TS Set A, 96 rxn
Core Slides and Reagents
1000520
Visium CytAssist Spatial Gene Expression for FFPE, Human Transcriptome, 6.5mm, 4 rxns
1000443
Visium CytAssist Spatial Gene Expression for FFPE, Human Transcriptome, 6.5mm, 16 rxns
1000522
Visium CytAssist Spatial Gene Expression for FFPE, Human Transcriptome, 11mm, 2 rxns
1000444
Visium CytAssist Spatial Gene Expression for FFPE, Human Transcriptome, 11mm, 8 rxns
1000521
Visium CytAssist Spatial Gene Expression for FFPE, Mouse Transcriptome, 6.5mm, 4 rxns
1000445
Visium CytAssist Spatial Gene Expression for FFPE, Mouse Transcriptome, 6.5mm, 16 rxns
1000523
Visium CytAssist Spatial Gene Expression for FFPE, Mouse Transcriptome, 11mm, 2 rxns
1000446
Visium CytAssist Spatial Gene Expression for FFPE, Mouse Transcriptome, 11mm, 8 rxns
Starter Kits and Accessories
1000469
Visium 6.5mm Slide Cassette, 4 pk
1000470
Visium 11mm Slide Cassette, 4 pk
1000471
Visium CytAssist Tissue Slide Cassette, 4 pk, 6.5 mm
1000472
Visium CytAssist Tissue Slide Cassette, 4 pk, 11 mm
RNAase Inhibitor (Optional)
1000887
Kit, RNase Inhibitor 40x
Documents and resources
User guides
Technical notes
Sequencing requirements
The final gene expression library is sequenced at a recommended minimum depth of 25K read pairs per capture spot covered by tissue, and the final protein expression library is sequenced at a recommended depth of 5K read pairs per capture spot covered by tissue.
View moreData analysis
Space Ranger Analysis Pipelines
Free analysis pipelines that process the input file types to align Visium sequencing data with the tissue image.
Learn about Space Ranger
Loupe Browser
Desktop application for Windows and MacOS that allows you to interactively visualize data.
Download Loupe BrowserFeatured datasets
Visium CytAssist Gene and Protein Expression Library of Human Tonsil with Add-on Antibodies, H&E, 6.5 mm (FFPE)
View datasetVisium CytAssist, Mouse Embryo, 11 mm Capture Area (FFPE)
View datasetVisium CytAssist Gene Expression Libraries of Post-Xenium Human Colon Cancer (FFPE)
View datasetRelated products
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A robust and sensitive probe-based solution for whole transcriptome spatial gene expression at single cell scale
Visium HD 3’
Versatility meets resolution in our species agnostic Visium HD assay, offering 3’ whole transcriptome spatial gene expression at single cell scale
Visium v1 3’
Our original 3' poly(A) whole transcriptome spatial gene expression assay