Perform whole transcriptome gene expression analysis at single cell scale with continuous tissue coverage.
Leverage precise transcript localization enabled by Visium CytAssist to generate the highest quality spatial transcriptomics data.
Generate powerful data from human or mouse fresh frozen, fixed frozen, and FFPE samples, including archived blocks or pre-sectioned slides.
Enhance your H&E and IF imaged samples with high-resolution spatial transcriptomics from the same tissue section.
Next-generation slide architecture enables single cell–scale resolution
Visium HD Spatial Gene Expression slides contain two 6.5 x 6.5 mm Capture Areas with a continuous lawn of oligonucleotides arrayed in millions of 2 x 2 µm barcoded squares without gaps, achieving single cell–scale spatial resolution. The data is output at 2 µm, as well as multiple bin sizes. The 8 x 8 µm bin is the recommended starting point for visualization and analysis.
- Follow standard histology practices to section, stain, and image tissues directly on glass slides.
- Pre-screen to find the most biologically significant tissue sections.
- Explore diverse FFPE, fresh frozen, and fixed frozen tissues with no optimization required.
Visium CytAssist
Unmatched spatial data quality with a simple workflow
Easy-to-use visualization tools seamlessly combine histological and gene expression data
Explore gene expression clusters in the tissue context
Map gene expression onto your H&E or IF image
Identify gene signatures of neighboring regions
Proven results
Videos
Resources
Visium HD enables spatial discovery in FFPE human breast cancer at single-cell scale
Visium HD enables spatial discovery in FFPE human breast cancer at single-cell scale
Poster, 10x Genomics
Poster, 10x Genomics
Grant application resources for Visium Spatial products
Grant application resources for Visium Spatial products
Grant Application Resources, 10x Genomics
Grant Application Resources, 10x Genomics
Whole transcriptome spatial discovery at the resolution you need
Whole transcriptome spatial discovery at the resolution you need
Flyer, 10x Genomics
Flyer, 10x Genomics
Frequently asked questions
Visium HD Spatial Gene Expression slides contain two 6.5 x 6.5 mm Capture Areas with a continuous lawn of oligonucleotides arrayed in millions of 2 x 2 µm barcoded squares without gaps, achieving single cell–scale spatial resolution.
Visium HD is compatible with human and mouse fresh frozen, fixed frozen, and formalin-fixed paraffin-embedded (FFPE) tissue sections containing mRNA. This includes archived FFPE blocks and pre-sectioned tissue on glass slides.
Visium HD Spatial Gene Expression is compatible with hematoxylin and eosin (H&E) staining for morphological information and with immunofluorescence (IF) staining for protein co-detection.
10x Genomics provides two types of software to help you analyze your data: Space Ranger and Loupe Browser. Space Ranger is an analysis software that automatically overlays spatial gene expression information on your tissue image and identifies clusters of barcoded squares with similar transcription profiles. You can then use Loupe Browser, a visualization software, to interactively explore the results. You can download Space Ranger and Loupe Browser from the 10x Genomics Support site at no cost.
Visium Spatial Platform
Bringing spatial context to unbiased whole transcriptome profiling, Visium was designed to help scientists map the relationships between cellular identity, function, interactions, and location in tissue architecture.