Visium CytAssist

Bridging histology and genomics

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Visium CytAssist instrument


  • Choice

    Start from FFPE blocks, or pre-sectioned FFPE or fresh frozen (FF) tissues on glass slides

  • Confidence

    Pre-screen to find the most biologically relevant tissue sections.

  • Flexibility

    Integrate seamlessly with standard histology workflows for H&E and IF staining.

Powerful platform for spatial discovery

  • The Visium CytAssist is a compact instrument designed to simplify the Visium workflow by facilitating the transfer of transcriptomic probes from standard glass slides to Visium slides, enabling whole transcriptomic spatial profiling insights across your entire tissue section, and expanded compatibility with FFPE and/or FF samples.
    CytAssist instrument with labeled dimensions, 10" x 8" x 12"
  • Simplified sample management

    It all starts with a slide. The Visium CytAssist enables users to start with tissue on standard glass slides, allowing input tissue to be prepared, stained, and imaged using standard histology workflows.

  • Expanded sample access

    Compatible with H&E or immunofluorescence stained FFPE or FF tissue sections, the CytAssist instrument allows you to use pre-sectioned and pre-stained tissues with 6.5 x 6.5 mm or 11 x 11 mm Visium Capture Area slides.

  • Maximized spatial insights

    Prescreen tissue sections, and then use the instrument for precise manual alignment of tissue section onto Visium Capture Areas, enabling you to see more of the tissue you care about.

Visium workflow with CytAssist

Visium for FFPE workflow, including transfer of sample from standard glass slide to Visium slide using CytAssist

Facilitate transfer of transcriptomic probes in FFPE or fresh frozen samples with Visium CytAssist. In the Visium CytAssist workflow, sectioning, tissue preparation, staining (H&E or IF), and imaging take place on a standard glass slide. After probe hybridization, two standard glass slides and a Visium slide with two Capture Areas are placed in the CytAssist instrument so that the tissue sections on the standard slides can be aligned on top of the Capture Areas. Within the instrument, a brightfield image is captured to provide spatial orientation for data analysis, followed by hybridization of transcriptomic probes to the Visium slide. The remaining steps, starting with probe extension, follow the standard Visium workflow outside of the instrument.

10x Visium Discovery Hub

Explore the power of spatial biology

Explore Visium datasets generated from FFPE and FF tumor samples, and experience what spatially resolved biology can do for you.

Illustration of a human body in interactive Visium Discovery Hub

Visium CytAssist

A compact instrument to facilitate the transfer of transcriptomic probes from sectioned tissues to Visium Slides.

Visium gene expression reagents

Compatible with Visium Spatial Gene Expression.

Data visualization software

Our analysis pipelines

Our visualization software

World-class technical and customer support

Contact our support team by phone or email.

Frequently Asked Questions

Visium CytAssist enables access to fresh frozen sections or FFPE tissues archived in blocks or pre-sectioned onto standard glass slides. Existing samples can be stained with H&E staining or IF antibodies on the glass slides before introduction to the Visium CytAssist workflow.

The CytAssist instrument uses kits with a probe-based chemistry compatible with human and mouse FFPE or fresh frozen samples.

No. The existing Visium CytAssist Spatial Gene Expression for FFPE reagent and slide kits are compatible with both fresh frozen and FFPE tissue samples sectioned onto standard glass slides

Bridge histology and genomics with Visium CytAssist