Highly sensitive detection of 5’ mRNA with antigen specificity and phenotyping.
Complete immune repertoire
Paired, full-length Ig sequence, including isotypes, and T-cell receptor α/β.
Combine immune repertoire with antigen specificity at single cell resolution.
Streamlined data analysis
Explore and visualize clonotype information with easy-to-use software.
One-day lab workflow
From sample to sequencing-ready library in one day.
Diverse sample compatibility
Demonstrated on PBMCs, fresh and frozen cell samples, and dissociated tissue.
Explore what you can do
Discover novel cell types and functions
Recover a comprehensive immune repertoire
Explore immune receptor mapping
Discover new biomarkers
Flexible Targeted and Whole Transcriptome Assays
Explore immune cell diversity through single cell whole transcriptome analysis or focus on your genes of interest using targeted gene expression panels.
Targeted Gene Expression Panels
Choose from pre-designed immunology, cancer, neuroscience, and gene signature panels with the ability to add up to 200 custom genes, or design fully custom panels.
With our comprehensive solutions you get:
Create foundational immune cell atlases, discover new cell types and states, or fully characterize antigen-binding cells with whole transcriptome analysis.
Validate discoveries or explore new hypotheses with the ability to transition seamlessly between whole transcriptome and targeted analysis from the same library preparation.
More for less
Scale your studies or maximize your budget using targeted panels to decrease sequencing costs.
Capabilities for multiomic readouts
Immune Repertoire Profiling
Understand clonal expansion and diversity by capturing full-length, paired B-cell and T-cell receptors at a single cell level.
- Paired receptor sequence enables reexpression and testing for antigen binding and function
- Combined with gene expression data, perform immune cell classification and discover new cell states
Feature Barcode Technology
Cell Surface Protein
Correlate gene expression measurements with immunophenotyping at a single cell level to better delineate immune cell subsets and functional states.
- Enable ultra-high parameter discovery, using multiomic cytometry to detect up to hundreds of proteins simultaneously with unbiased gene expression profiling
- Stratify immune cell populations by correlating protein isoforms with regulatory network co-expression in single cells
Feature Barcode Technology
Assess the specificity of the adaptive immune system at single cell resolution with simultaneous capture of paired T cell receptors and peptide-MHC multimers, or immunoglobulin heavy and light chains and their cognate antigens.
- Explore public and private T-cell antigen specificities using barcoded peptide-MHC multimers
- Characterize how neoantigens may be targeted by the adaptive immune system
- Accelerate antibody discovery with a faster and higher throughput screening technology
Watch how customers are using Chromium Single Cell Immune Profiling.
Search hundreds peer-reviewed publications using Chromium Single Cell Immune Profiling.
Find the latest app notes and other documentation Chromium Single Cell Immune Profiling.
Our End-to-End Solution
Chromium Instrument with Next GEM technology
Our compact instrument
Chromium Single Cell Immune Profiling Reagents
With our reagent kits, explore adaptive immunity through multidimensional approaches, including 5’ mRNA, full-length paired BCR and TCR sequences, antigen-receptor specificity, cell surface proteins, and more.
Analysis and Visualization Software
Our analysis pipelines
Our visualization software
World-Class Technical and Customer Support
Our expert support team can be contacted by phone or email.
The base workflow for Single Cell Immune Profiling. If you’re using our Feature Barcoding Kit for Cell Surface Protein or Antigen Specificity, we provide additional protocols for modifying your cells of interest.
Prepare your sample
Start with hundreds to thousands of cells. Cells can be labeled with antibodies and/or peptide-MHCs conjugated with a unique oligonucleotide barcode. You can use cell suspensions from FACS/MACS, fine needle aspirates, TILs, PBMCs, and more.Resources
Construct your 10x library
Construct a 10x barcoded library using our reagent kits and Chromium Controller. The Chromium Controller encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within each nanoliter-scale partition, cells undergo reverse transcription to generate cDNA, which shares a 10x barcode with all cDNA from its individual cell of origin.Resources
The resulting 10x Barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers, for immune and transcriptional profiling of thousands of individual cells.Resources
Analyze Your Data
Convert the raw sequencing data to biologically meaningful insights with Cell Ranger. Cell Ranger processes data from paired immune receptor repertoire sequences, gene expression, and antigen specificity.
Analysis pipelines output
Output includes QC information and files that can be easily used for further analysis in our Loupe Browser visualization software, or third-party R or Python tools.Resources
Visualize Your Data
Use our Loupe Browser visualization software to interactively explore your results and compare detailed clonotype information within and across samples and cell types to study gene usage, repertoire diversity, clonal expansion, and more.
Do I need to be a bioinformatician to use it?
Loupe is a point-and-click software that’s easy for anyone to download and use.Resources
Frequently Asked Questions
The solution is capable of the modular integration of multiple data types, including mRNA-based whole transcriptome or targeted gene expression, immunophenotyping, adaptive immune repertoire clonality and diversity, and antigen specificity, simultaneously from the same single cells. This multiomic approach to cell characterization helps decipher the rules of the immune system one cell at a time.
Yes. You can amplify only the immunoglobulin or TCR libraries for sequencing and save the amplified whole transcriptome cDNA material for later sequencing.
Chromium Single Cell Immune Profiling is compatible with fresh or cryopreserved single cell suspensions. Typical samples include PBMCs, tumor-infiltrating lymphocytes, spleen, bone marrow cells, and other tissue types. Species can range from human, mouse, rat, and other model organisms. However, only human and mouse are currently supported by 10x Genomics reagents and protocols for immunoglobulin or TCR sequencing.
The Chromium Single Cell Immune Profiling Solution makes use of gene-specific amplification primers that target the constant regions of Ig and TCR genes in order to enrich and sequence across the entire Variable, Diversity, and Joining gene segments.
Yes, immunoglobulin heavy chain sequences are classified by IgA, IgD, IgE, IgM, and IgG.