Universal 5'

Up to 80% cell recovery
Up to 100% higher sensitivity vs prior Next GEM tech
More usable reads, save up to 50% on sequencing costs
Why choose Universal 5'?
Require full-length transcript readouts
Flexible throughput from 500 to 20K cells per sample
Broad multiomic options including protein, CRISPR, gene expression, TCR/BCR, and antigen specificity
Assay specifications
- Mouse
- Human
- Rat (except with TCR/BCR)
- Non-human primate (except with TCR/BCR)
- and more
Species
- Cell suspension
- Nuclei suspension
- Fresh tissue
- Frozen tissue
- Flow-sorted cells
- Fixed human PBMCs
- Organoids
Compatible samples
- 5' gene expression
- Cell surface protein
- TCR/BCR sequencing
- CRISPR screening
- Antigen specificity
Multiomics
Compatible instruments
- 20K cells/sample
- 160K cells/chip
- 8 samples/chip
Singleplex throughput options
- 5K cells/sample
- 40K cells/chip
- Up to 4 samples/channel output, 8 samples/chip
On-chip multiplexing throughput options (New!)
Shop reagents, kits & more
Universal 5' Gene Expression
Each 5' experiment requires a chip, index kit, and core reagents plus optional V(D)J amplification, CRISPR, or feature barcoding kits. If you're unsure what you need, use the experiment builder to find the custom list of items required for your experiment.

Documents and resources
Videos
Data analysis
Cell Ranger
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data
Loupe Browser
Desktop application for Windows and MacOS that allows you to interactively visualize data.
Cloud Analysis
Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser
Featured publications
Publications
Nature Medicine 2023 Jun Tian, et al.
Nature Cancer, 2023 Francesco Maura, et al.
Nature Medicine, 2023 Nathaniel Anderson, et al.