Visium Spatial Platform

Whole transcriptome spatial discovery

10x Genomics Visium platform

Best-in-class spatial data quality with complete coverage

The Visium platform delivers unbiased, whole transcriptome spatial gene expression analysis at single cell scale with unmatched spatial data quality and offers a flexible assay portfolio to study a broad range of tissues.

10x Genomics Visium platform

Visium slides and reagents

High-resolution, whole transcriptome spatial gene expression across species and tissue conditions

Visium CytAssist instrument

Enables a histology-friendly workflow and provides high spatial fidelity

Data analysis

Free, easy-to-use software for intuitive data visualization and exploration

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NEW! Visium HD 3' assay

Deeper spatial discovery

  • Species agnostic, whole transcriptome spatial analysis at true single cell resolution with cell segmentation
  • De novo discovery, including the ability to detect isoforms, TCRs/BCRs, and SNVs
  • High spatial fidelity and a streamlined, histology-friendly workflow powered by the Visium CytAssist instrument

Whole transcriptome spatial biology at the resolution you need

Our Visium HD product line offers a distinct slide architecture and two chemistries to explore the whole transcriptome spatial biology of your samples at the resolution you need.

Visium HD assays icon

Visium HD WT Panel

  • Recommended for differential gene expression in human and mouse
Protein coding gene coverage
  • Optimized for archival and FFPE samples
Flexible tissue compatibility
  • Compatible with FFPE, fresh frozen, and fixed frozen tissues
Species-agnostic Visium HD 3'

NEW! Visium HD 3'

  • Recommended for expanded discovery applications
Whole transcriptome coverage
  • Delivers broadest set of information, including isoforms, SNPs, etc.
Explore across species
  • Tested in human, mouse, rat, pig, non-human primate and more.

Unmatched spatial data quality with a histology-friendly workflow

  • Ensure high spatial fidelity through precise transcript localization facilitated by the Visium CytAssist instrument
  • Leverage a standard histological workflow to section, stain, and image tissues directly on glass slides
  • Pre-screen and orient tissue sections to align the most desired region of tissue onto the Capture Area of the Visium slide
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Accelerate spatial discovery with intuitive data exploration

Screenshot of Cloud Analysis

Simple, fast analysis with Space Ranger

  • Upload FASTQ files to 10x Cloud Analysis or download our free software for local analysis
  • Process spatial sequencing data with imaging data in minutes, no command line required

Immediate insights with powerful data visualization

  • Visualize and explore thousands of spatially variable genes in a few clicks with Loupe Browser
  • Explore gene expression clusters in the tissue context to define cell types or gene signatures by region and morphology

Resources

Screenshot of Loupe browser

See Visium HD data in action

Learn how to map the tumor microenvironment of colorectal cancer with the Visium HD assay and Loupe Browser in this guided data tutorial.

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Grant writing assistance

Get the information you need to write grants featuring Visium experiments (including technology overview, benchmarking data, and more).

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Spatial publications

Browse peer-reviewed publications to see how researchers have used Visium Spatial technology to accelerate novel discoveries.

Unlock the full spectrum of biology

Elevate your Visium Spatial insights with comprehensive single cell analysis or targeted, precision insights into the tissue context.

Platform

Chromium Single Cell platform

Chromium Single Cell platform

Visium Spatial platform

Visium Spatial platform

Xenium Spatial platform

Xenium Spatial platform

When to use

Comprehensive single cell data

Ideal for deep characterization of cell populations and states.

High-resolution spatial gene expression

Understand complex tissues, neighborhoods, and cell to cell interactions. Integration with other spatial-omics, histology, and morphology. 

Why to use

Unbiased single cell discovery

High per-gene sensitivity

Unbiased spatial discovery

Targeted spatial exploration

High per-gene sensitivity

Applications

Whole transcriptome gene expression

Protein

TCR, BCR

CRISPR

ATAC

Whole transcriptome gene expression

Targeted gene expression (up to 5,000 genes)

Resolution

Single cell

Transcripts assigned to 2-µm areas

Single cell

Data readout

NGS-based

NGS-based

Imaging-based

Sample compatibility

Single cell or nuclei suspensions from fresh, frozen, or FFPE samples

FFPE

Fresh frozen

Fixed frozen

Fresh frozen

FFPE

Start your Visium journey