Consistent results
Reduce variability in single cell data, generating reproducible and consistent results.
Maximum lab productivity
Free up hands-on time for other experiments with walk-away convenience for single cell workflows.
Integrated and validated
Automate single cell partitioning, barcoding, and library prep with one optimized instrument.
Many single cell assays
Efficiently characterize single cells with gene expression or multiomic immune profiling.
One-day automated workflows
Go from sample to sequencing-ready libraries in one day, with flexible, modular workflow options.
World-class technical support
Rely on expert on-site instrument installation and remote troubleshooting technical assistance.
10x Your Productivity
One system, many single cell assays
With Automation for Single Cell Gene Expression, you can automate your 3’ gene expression workflows to explore cellular heterogeneity, discover novel targets and biomarkers, and unravel complex cellular processes, with increased productivity. With Automation for Single Cell Immune Profiling workflows, you can automate your 5’ gene expression and V(D)J amplification workflows to examine cellular heterogeneity of the immune system and T- and B-cell receptor diversity, at scale. Automated workflows for 5' multiomic profiling of single cell gene expression and cell surface protein are also available.
Reduce technical variability
Chromium Connect delivers consistent and reproducible single cell data across experiments and across users. Run your assays with the confidence that technical variation is minimized with this validated instrument.
Save time and resources
Chromium Connect reduces errors from manual pipetting and is estimated to shorten your hands-on time from more than 8 hours to less than an hour. Use this automated solution to get sequencing-ready libraries every day and reclaim valuable lab time.
Compare automated and manual workflows
Consistent and reproducible library prep
Chromium Connect integrates components customized for the 10x Genomics single cell workflows, automation-specific reagents and consumables, and an easy-to-use touchscreen computer. Generate consistent and reproducible single cell gene expression results across experiments, across users, and even across multiple sites.
Generating Consistent Single Cell Gene Expression Libraries with an Automated Workflow
Chromium Automated Controller
Inside Chromium Connect, the compact Chromium Automated Controller performs single cell partitioning and barcoding.- Integrate and automate single cell partitioning
- Minimize new workflow training
- Reduce hands-on time
Automation-specific single cell reagents
Chromium Connect and automation-specific reagents are designed for error-proof reagent loading.- Minimize waste
- Enable reagent and lot tracking
- Ensure proper system loading
Guided touchscreen operation
Simple operation via a clean touchscreen interface guides the user through each step of the experimental setup.- Step-by-step guidance
- Minimize user interaction
- LIMS compatible
Proven Results
Proven Results
Resources
Find the latest app notes and other documentation for Chromium Connect
Chromium Connect: Modular Gene Expression Workflow & Data Overview
Chromium Connect: Modular Gene Expression Workflow & Data Overview
Technical Note, 10x Genomics
Technical Note, 10x Genomics
Chromium Connect: Consistent Automated Single Cell 5' Gene Expression and V(D)J Library Generation
Chromium Connect: Consistent Automated Single Cell 5' Gene Expression and V(D)J Library Generation
Technical Note, 10x Genomics
Technical Note, 10x Genomics
Generating Consistent Single Cell Gene Expression Libraries with an Automated Workflow
Generating Consistent Single Cell Gene Expression Libraries with an Automated Workflow
White Paper, 10x Genomics
White Paper, 10x Genomics
Our End-to-End Solution
Chromium instruments with Next GEM technology
Our automated instrument
Our compact instrument
Automation-specific single cell reagents
Automation-specific reagents come in pre-aliquoted strip tubes, with flexibility to run 1–8 samples without wasted reagents.
Analysis and visualization software
Convert your data to biologically meaningful insights and interactively explore your results with easy-to-use software.
World-class technical and customer support
Our expert support team can be contacted by phone or email.
Frequently Asked Questions
Chromium Connect supports automated workflows for single cell gene expression and single cell immune profiling, including T- and B-cell receptor profiling. Automated workflows for multiomic profiling of 5' gene expression, cell surface proteins, and antigen specificity are also available. Chromium Next GEM Automated Single Cell reagent kits include single-use, automation-specific reagents that come in pre-aliquoted strip tubes with the flexibility to run 1–8 samples, without wasted reagents. These reagents are color-coded and 2D-barcoded for error-proof reagent loading and tracking.
The data is at parity with manual single cell assay results, and shows reproducibility and consistency from channel to channel and instrument to instrument. For more information, refer to the Technical Notes, Chromium Connect: Consistent Automated Single Cell Gene Expression Library Generation (CG000284), Chromium Connect: Consistent Automated Single Cell 5’ Gene Expression and V(D)J Library Generation (CG000385), and Chromium Connect: Modular Gene Expression Workflow & Data Overview (CG000484).
Chromium Connect provides end-to-end workflows from sample input to library generation. All library preparation steps from GEM generation, GEM-RT, cDNA amplification and cleanup, and library construction are performed on the instrument deck.
Modular workflows include safe stopping points after cDNA generation, offering the convenience to batch samples for library construction. Users can also choose to perform automated library construction starting with purified cDNA generated manually on the Chromium X/iX or Controller, or from prior Connect runs.
In addition, other automated workflow options include TCR or BCR amplification and 5' Feature Barcode reactions for detecting cell surface proteins and antigen specificity, as well as optional assays such as qPCR setup and pooling.
The instrument price includes installation, training, 6-month preventative maintenance, and a 12-month warranty on the instrument.
No, Chromium Connect software has been specifically designed for use with our highly optimized automated 10x Genomics protocols.
Compatible Products
Single Cell Gene Expression
Profile cell types and states with 3' gene expression, surface protein expression, and CRISPR perturbations in the same cell.
Single Cell Immune Profiling
Analyze full length paired BCRs/TCRs, surface protein expression, antigen specificity, and 5' gene expression in the same cell.