Single Cell Methylation

Detection of differentially methylated regions

Massively parallelized single cell barcoding

Target enrichment compatibility

Why choose Single Cell Methylation?

Interrogate hundreds of thousands of CpG and CH sites per cell
Capture thousands of cells per run with seamless sample multiplexing
Maximize your sequencing budget by focusing on specific regions of interest

Assay specifications

Method

DNA methylation

Consider this kit for

Small kit:
Oncology, neuroscience, and aging studies; capturing cell type–specific epigenetic signatures

Large kit:
High-throughput oncology, neuroscience, and aging studies; capturing cell type–specific epigenetic signatures

Sample types

Nuclei from cell lines, fresh and frozen tissue, across multiple species

Number of samples (with multiplexing)

Small kit:
Built-in sample pooling allows multiplexing of up to 6 samples per run

Large kit:
Built-in sample pooling allows multiplexing of up to 8 samples per run (with additional purchase of nuclei resuspension kit)

Number of nuclei

Small kit:
~4,100

Large kit:
~16,400

Chemistry

Massively parallelized combinatorial barcoding with a demonstrated low doublet rate of 8%

Compatible flowcells

NextSeq 1000/2000, NovaSeq6000, and NovaSeqX

Sequencing requirements

Whole genome: 2M reads/cell
Target enriched (e.g., Twist Human Methylome Panel): 500K reads/cell

Key advantages

Epigenetics at single cell resolution

Profile methylation state, a heritable and stable mark ideal for robust detection of epigenetic complexity in tissues.

Cell type–specific methylation patterns

Break through the noise of bulk-level analysis, and unmask methylation patterns specific to different cell types.

Target enrichment capabilities

Maximize your sequencing budget and profile more samples with target-enriched libraries, without worrying about leaving important CpGs behind.

Streamlined protocols

Run simplified workflows, powered by massively parallelized barcoding, powerful technology that leverages the cell as the reaction compartment.

High-throughput, kitted solution

Detect single cell methylomes from thousands of cells with our hassle-free, kitted solution, no custom protocols or unvalidated reagents needed.

Shop reagents, kits & more

Scale Single Cell

Methylation

Each single cell Methylation experiment requires a nuclei resuspension kit and core reagent. If you're unsure what you need, please visit Methylation product page or contact your local 10x sales representative for more information.

Build your own Single Cell Methylation experiment

Design the experiment that's right for you

Start building

Resources