Chromium Single Cell Immune Profiling

Multiomic Profiling of Immune Diversity, Cell by Cell

Chromium Single Cell Immune Profiling provides a multiomic solution to your immunology questions. Analyze full length paired B-cell or T-cell receptors, surface protein expression, antigen specificity, and gene expression, all from a single cell.

Single Cell Immune Profiling
  • Multiomic cytometry

    Multiomic cytometry

    Highly sensitive detection of 5’ mRNA with antigen specificity and phenotyping.

  • Complete immune repertoire

    Complete immune repertoire

    Paired, full-length Ig sequence, including isotypes, and T-cell receptor α/β.

  • Immune mapping

    Immune mapping

    Combine immune repertoire with antigen specificity at single cell resolution.

  • Streamlined data analysis

    Streamlined data analysis

    Explore and visualize clonotype information with easy-to-use software.

  • One-day lab workflow

    One-day lab workflow

    From sample to sequencing-ready library in one day.

  • Diverse sample compatibility

    Diverse sample compatibility

    Demonstrated on PBMCs, fresh and frozen cell samples, and dissociated tissue.

Explore what you can do

  • Discover novel cell types and functions

  • Recover a comprehensive immune repertoire

  • Explore immune receptor mapping

  • Discover new biomarkers

Flexible Targeted and Whole Transcriptome Assays

Explore immune cell diversity through single cell whole transcriptome analysis or focus on your genes of interest using targeted gene expression panels.

Targeted Gene Expression Panels

Choose from pre-designed immunology, cancer, neuroscience, and gene signature panels with the ability to add up to 200 custom genes, or design fully custom panels.

With our comprehensive solutions you get:

Powerful discovery

Create foundational immune cell atlases, discover new cell types and states, or fully characterize antigen-binding cells with whole transcriptome analysis.

Seamless integration

Validate discoveries or explore new hypotheses with the ability to transition seamlessly between whole transcriptome and targeted analysis from the same library preparation.

More for less

Scale your studies or maximize your budget using targeted panels to decrease sequencing costs.

Capabilities for multiomic readouts

Immune Repertoire Profiling

Immune Repertoire Profiling

Understand clonal expansion and diversity by capturing full-length, paired B-cell and T-cell receptors at a single cell level.

  • Paired receptor sequence enables reexpression and testing for antigen binding and function
  • Combined with gene expression data, perform immune cell classification and discover new cell states
Cell Surface Protein

Feature Barcode Technology

Cell Surface Protein

Correlate gene expression measurements with immunophenotyping at a single cell level to better delineate immune cell subsets and functional states.

  • Enable ultra-high parameter discovery, using multiomic cytometry to detect up to hundreds of proteins simultaneously with unbiased gene expression profiling
  • Stratify immune cell populations by correlating protein isoforms with regulatory network co-expression in single cells
Antigen Specificity

Feature Barcode Technology

Antigen Specificity

Assess the specificity of the adaptive immune system at single cell resolution with simultaneous capture of paired T cell receptors and peptide-MHC multimers, or immunoglobulin heavy and light chains and their cognate antigens.

  • Explore public and private T-cell antigen specificities using barcoded peptide-MHC multimers
  • Characterize how neoantigens may be targeted by the adaptive immune system
  • Accelerate antibody discovery with a faster and higher throughput screening technology

Proven Results

Proven Results

Videos

Watch how customers are using Chromium Single Cell Immune Profiling.

Publications

Search hundreds peer-reviewed publications using Chromium Single Cell Immune Profiling.

Resources

Find the latest app notes and other documentation Chromium Single Cell Immune Profiling.

Our End-to-End Solution

Chromium Instrument with Next GEM technology

Chromium Instrument with Next GEM technology

Our compact instrument

Chromium Single Cell Immune Profiling Reagents

Chromium Single Cell Immune Profiling Reagents

With our reagent kits, explore adaptive immunity through multidimensional approaches, including 5’ mRNA, full-length paired BCR and TCR sequences, antigen-receptor specificity, cell surface proteins, and more.

Analysis and Visualization Software

Analysis and Visualization Software

Our analysis pipelines

Our visualization software

World-Class Technical and Customer Support

World-Class Technical and Customer Support

Our expert support team can be contacted by phone or email.

Workflow

The base workflow for Single Cell Immune Profiling. If you’re using our Feature Barcoding Kit for Cell Surface Protein or Antigen Specificity, we provide additional protocols for modifying your cells of interest.

  1. 1Step 1

    Prepare your sample

    Start with hundreds to thousands of cells. Cells can be labeled with antibodies and/or peptide-MHCs conjugated with a unique oligonucleotide barcode. You can use cell suspensions from FACS/MACS, fine needle aspirates, TILs, PBMCs, and more.

    Resources
  2. 2Step 2

    Construct your 10x library

    Construct a 10x barcoded library using our reagent kits and Chromium Controller. The Chromium Controller encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within each nanoliter-scale partition, cells undergo reverse transcription to generate cDNA, which shares a 10x barcode with all cDNA from its individual cell of origin.

    Resources
  3. 3Step 3

    Sequence

    The resulting 10x Barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers, for immune and transcriptional profiling of thousands of individual cells.

    Resources
  4. 4Step 4

    Analyze Your Data

    Convert the raw sequencing data to biologically meaningful insights with Cell Ranger. Cell Ranger processes data from paired immune receptor repertoire sequences, gene expression, and antigen specificity.

    Analysis pipelines output

    Output includes QC information and files that can be easily used for further analysis in our Loupe Browser visualization software, or third-party R or Python tools.

    Resources
  5. 5Step 5

    Visualize Your Data

    Use our Loupe Browser visualization software to interactively explore your results and compare detailed clonotype information within and across samples and cell types to study gene usage, repertoire diversity, clonal expansion, and more.

    Do I need to be a bioinformatician to use it?

    Loupe is a point-and-click software that’s easy for anyone to download and use.

    Resources

Frequently Asked Questions

The solution is capable of the modular integration of multiple data types, including mRNA-based whole transcriptome or targeted gene expression, immunophenotyping, adaptive immune repertoire clonality and diversity, and antigen specificity, simultaneously from the same single cells. This multiomic approach to cell characterization helps decipher the rules of the immune system one cell at a time.

Yes. You can amplify only the immunoglobulin or TCR libraries for sequencing and save the amplified whole transcriptome cDNA material for later sequencing.

Chromium Single Cell Immune Profiling is compatible with fresh or cryopreserved single cell suspensions. Typical samples include PBMCs, tumor-infiltrating lymphocytes, spleen, bone marrow cells, and other tissue types. Species can range from human, mouse, rat, and other model organisms. However, only human and mouse are currently supported by 10x Genomics reagents and protocols for immunoglobulin or TCR sequencing.

The Chromium Single Cell Immune Profiling Solution makes use of gene-specific amplification primers that target the constant regions of Ig and TCR genes in order to enrich and sequence across the entire Variable, Diversity, and Joining gene segments.

Yes, immunoglobulin heavy chain sequences are classified by IgA, IgD, IgE, IgM, and IgG.

Single Cell Gene Expression

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