Chromium Single Cell Immune Profiling

Multiomic Profiling of Immune Diversity, Cell by Cell

Chromium Single Cell Immune Profiling provides a multiomic solution to your immunology questions. Analyze full length paired B-cell or T-cell receptors, surface protein expression, antigen specificity, and gene expression, all from a single cell.

Single Cell Immune Profiling

Multiomic cytometry

Highly sensitive detection of 5’ mRNA with antigen specificity and phenotyping.

Complete immune repertoire

Paired, full-length Ig sequence, including isotypes, and T-cell receptor α/β.

Immune mapping

Combine immune repertoire with antigen specificity at single cell resolution.

Streamlined data analysis

Explore and visualize clonotype information with easy-to-use software.

One-day lab workflow

From sample to sequencing-ready library in one day.

Diverse sample compatibility

Demonstrated on PBMCs, fresh and frozen cell samples, and dissociated tissue.

Explore what you can do

  • Discover novel cell types and functions

  • Recover a comprehensive immune repertoire

  • Explore antigen specificity

  • Discover new biomarkers

Flexible Targeted and Whole Transcriptome Assays

Explore immune cell diversity through single cell whole transcriptome analysis or focus on your genes of interest using targeted gene expression panels.

Targeted Gene Expression Panels

Choose from pre-designed immunology, cancer, neuroscience, and gene signature panels with the ability to add up to 200 custom genes, or design fully custom panels.

With our comprehensive solutions you get:

Powerful discovery

Create foundational immune cell atlases, discover new cell types and states, or fully characterize antigen-binding cells with whole transcriptome analysis.

Seamless integration

Validate discoveries or explore new hypotheses with the ability to transition seamlessly between whole transcriptome and targeted analysis from the same library preparation.

More for less

Scale your studies or maximize your budget using targeted panels to decrease sequencing costs.

Capabilities for multiomic readouts

Immune Repertoire Profiling

Understand clonal expansion and diversity by capturing full-length, paired B-cell and T-cell receptors at a single cell level.

  • Paired receptor sequence enables reexpression and testing for antigen binding and function
  • Combined with gene expression data, perform immune cell classification and discover new cell states

Feature Barcode Technology

Cell Surface Protein

Correlate gene expression measurements with immunophenotyping at a single cell level to better delineate immune cell subsets and functional states.

  • Enable ultra-high parameter discovery, using multiomic cytometry to detect up to hundreds of proteins simultaneously with unbiased gene expression profiling
  • Stratify immune cell populations by correlating protein isoforms with regulatory network co-expression in single cells

Feature Barcode Technology

Antigen Specificity

Assess the specificity of the adaptive immune system at single cell resolution with simultaneous capture of paired T cell receptors and peptide-MHC multimers, or immunoglobulin heavy and light chains and their cognate antigens.

  • Explore public and private T-cell antigen specificities using barcoded peptide-MHC multimers
  • Characterize how neoantigens may be targeted by the adaptive immune system
  • Accelerate antibody discovery with a faster and higher throughput screening technology

Proven Results

Proven Results


Watch how customers are using Chromium Single Cell Immune Profiling.


Search hundreds peer-reviewed publications using Chromium Single Cell Immune Profiling.


Find the latest app notes and other documentation Chromium Single Cell Immune Profiling.

Our End-to-End Solution

Chromium Instrument with Next GEM technology

Chromium Instrument with Next GEM technology

Chromium Single Cell Immune Profiling Reagents

Chromium Single Cell Immune Profiling Reagents

    With our reagent kits, explore adaptive immunity through multidimensional approaches, including 5’ mRNA, full-length paired BCR and TCR sequences, antigen-receptor specificity, cell surface proteins, and more.

    Analysis and Visualization Software

    Analysis and Visualization Software

    World-Class Technical and Customer Support

    World-Class Technical and Customer Support

      Our expert support team can be contacted by phone or email.


      The base workflow for Single Cell Immune Profiling. If you’re using our Feature Barcoding Kit for Cell Surface Protein or Antigen Specificity, we provide additional protocols for modifying your cells of interest.

      1. 1

        Prepare your sample

        Start with hundreds to thousands of cells. Cells can be labeled with antibodies and/or peptide-MHCs conjugated with a unique oligonucleotide barcode. You can use cell suspensions from FACS/MACS, fine needle aspirates, TILs, PBMCs, and more.

      2. 2

        Construct your 10x library

        Construct a 10x barcoded library using our reagent kits and Chromium Controller. The Chromium Controller encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within each nanoliter-scale partition, mRNA from cells undergo reverse transcription to generate cDNA, where all cDNA from individual cells share a common 10x barcode.

      3. 3


        The resulting 10x barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers, for immune and transcriptional profiling of thousands of individual cells.

      4. 4

        Analyze Your Data

        Convert the raw sequencing data to biologically meaningful insights with Cell Ranger, downloadable for no additional cost. Cell Ranger processes data from heavy/light chain sequencing, gene expression, and antigen specificity.

        Analysis pipelines output

        Output includes QC information and files that can be easily used for further analysis in our Loupe visualization software, or third-party R, or Python tools.

      5. 5

        Visualize Your Data

        Use our Loupe Browser visualization software to interactively explore your results and compare detailed clonotype information within and across samples and cell types to study gene usage, repertoire diversity, clonal expansion and more.

        Do I need to be a bioinformatician to use it?

        Loupe is a point-and-click software that’s easy for anyone to download and use.


      Frequently Asked Questions

      The solution is capable of the modular integration of multiple data types, including mRNA-based whole transcriptome or targeted gene expression, immunophenotyping, adaptive immune repertoire clonality and diversity, and antigen specificity, simultaneously from the same single cells. This multiomic approach to cell characterization helps decipher the rules of the immune system one cell at a time.

      Yes. You can amplify only the immunoglobulin or TCR libraries for sequencing and save the amplified whole transcriptome cDNA material for later sequencing.

      Chromium Single Cell Immune Profiling is compatible with fresh or cryopreserved single cell suspensions. Typical samples include PBMCs, tumor-infiltrating lymphocytes, spleen, bone marrow cells, and other tissue types. Species can range from human, mouse, rat, and other model organisms. However, only human and mouse are currently supported by 10x Genomics reagents and protocols for immunoglobulin or TCR sequencing.

      The Chromium Single Cell Immune Profiling Solution makes use of gene-specific amplification primers that target the constant regions of Ig and TCR genes in order to enrich and sequence across the entire Variable, Diversity, and Joining gene segments.

      Yes, immunoglobulin heavy chain sequences are classified by IgA, IgD, IgE, IgM, and IgG.

      Related Products

      Single Cell Immune Profiling

      Single Cell Gene Expression

      Spatial Gene Expression

      Single Cell ATAC

      • Paired full-length TCR / BCR sequencing
      • 5’ whole transcriptome gene expression
      • Targeted gene expression
      • 3’ whole transcriptome gene expression
      • Targeted gene expression
      • 3’ transcriptome gene expression in tissues
      • Epigenomic landscape by open chromatin profiling
      • Cell Surface Protein
      • Antigen Specificity
      • Cell Surface Protein
      • CRISPR Screening
      Single Cell Immune Profiling

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