Chromium Single Cell

Flex Gene Expression

Probe-based assays using GEM-X technology to capture 18,000+ coding genes, customizable to fit project needs

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Exceptional performance with challenging samples

Reduced cell input recommendations: 25K

Why choose Flex Gene Expression

    Using archival or lower quality samples
    Need flexible sample batching
    Scale large projects for <1 cent per cell

Assay specifications

Species

Mouse
Human
Other species with custom probes

Compatible samples

Cell suspension
Nuclei suspension
Fresh tissue
Frozen tissue
FFPE tissue
PFA-fixed tissue
Flow-sorted cells
Fixed whole blood
Organoids

Multiomics

Probe-based gene expression
Cell surface protein
Intracellular protein
CRISPR screening

Compatible instruments

Chromium X

Throughput options

Singleplex

20K cells/sample
160K cells/chip
8 samples/chip

In-line multiplexing

20K cells/sample
2.56M cells/chip
128 samples/chip

Components

Each experiment requires a chip, index kit, and core reagents. If you're unsure what you need, use the experiment builder find the custom list of items required for your experiment.

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Consumables

Choose a version


Nuclei Isolation (optional)

REAGENT

1000494

Chromium Nuclei Isolation with RNase Inhibitor Kit, 16rxns

Sample Prep Kit

REAGENT

1000781

GEM-X Flex Sample Preparation v2 Kit, 48 rxns

Individual Wash Workflow (optional)

REAGENT

1000828

GEM-X Flex Supplemental Wash Kit, 64 rxns

Chip

CHIP

1000791

GEM-X Flex Gene Expression Chip Kit, 4 chips

Index Kit

REAGENT

1000251

Dual Index Kit TS Set A, 96 rxn

REAGENT

1000215

Dual Index Kit TT Set A 96 rxns

Core Reagents

REAGENT

1000792

GEM-X Flex Gene Expression Human, 4 samples

REAGENT

1000793

GEM-X Flex Gene Expression Human 4-plex, 16 samples

REAGENT

1000794

GEM-X Flex Gene Expression Human 16-plex, 64 samples

REAGENT

1000829

GEM-X Flex Gene Expression Human n-plex, 64 samples

REAGENT

1000795

GEM-X Flex Gene Expression Human 16-plex, 256 samples

REAGENT

1000796

GEM-X Flex Gene Expression Mouse, 4 samples

REAGENT

1000797

GEM-X Flex Gene Expression Mouse 4-plex, 16 samples

REAGENT

1000798

GEM-X Flex Gene Expression Mouse 16-plex, 64 samples

REAGENT

1000831

GEM-X Flex Gene Expression Mouse n-plex, 64 samples

REAGENT

1000799

GEM-X Flex Gene Expression Mouse 16-plex, 256 samples

Feature Barcoding Kit (optional)

REAGENT

1000628

Fixed RNA Feature Barcode Multiplexing Kit, 64 rxns

Compatible Instruments


Universal GEX
Build your own Flex Gene Expression experiment

Design the experiment that's right for you

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Documents and resources

View all technical documentation

Product sheet

Download product sheet

Sample prep

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User guides

Sequencing requirements

Minimum 10,000 read pairs/cell for gene expression; 5,000 read pairs/cell for protein analysis.

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Videos