Reveal the Full Complexity of Cellular Diversity with Single Cell Transcriptomics

Contact Me Request a Quote

The Chromium Single Cell Gene Expression Solution

Go Beyond Traditional Gene Expression Analysis

The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to tens of thousands of cells. Our latest improvements allow you to detect even more unique transcripts per cell and with the addition of Feature Barcoding technology, you can get a more complete molecular readout cell by cell—identify cell-specific CRISPR-mediated perturbations or simultaneously measure gene and cell surface protein expression in the same cell, with virtually unlimited possibilities for the additional feature types.

Go beyond traditional gene expression analysis to characterize cell populations, cell types, cell states, and more on a cell-by-cell basis. From assessing tumor heterogeneity and stem cell composition, to dissecting neuronal populations—the technological advancements provided by the Chromium Single Cell Gene Expression Solution, along with turnkey software tools, allow the creation of high complexity libraries from single cells to maximize insight from any sample type.

  • Identify and characterize rare cell types
  • Analyze and understand cellular heterogeneity and how this contributes to your biological system
  • Perform cellular phenotyping with single cell RNA-seq to identify novel targets, biomarkers, and cell types and states without the need for pre-selected targets
  • Evaluate mRNA and cell surface protein expression profiles within the same cell
  • Perform high throughput and high resolution functional genetic screens in tens of thousands of cells simultaneously
  • Assess comprehensive gene expression phenotypes for individual CRISPR perturbations
Gene Expression Profiling

Gene Expression Profiling

Uncover cell-to-cell gene expression variability and identify rare cell types in heterogeneous samples to better characterize cellular contributions during development and disease. Our latest improvements vastly increase sensitivity, so you can detect even more unique transcripts per cell.

Download Product Sheet
Feature Barcoding Technology: Cell Surface Protein

Feature Barcoding Technology: Cell Surface Protein

Better implement published methods like CITE-Seq/REAP-Seq with our new and improved workflow using Feature Barcoding technology. Seamlessly measure both gene and cell surface protein expression in the same cell to identify protein isoforms, detect protein for low abundance transcripts, and further increase phenotypic specificity.

Download Product Sheet
Antigen Specificity

Feature Barcoding Technology: CRISPR Screening

Identify CRISPR-mediated perturbations alongside cellular transcripts using Feature Barcoding technology. Implement this improved and novel methodology to conduct high-throughput and scalable functional genetic screens in hundreds to tens of thousands of single cells simultaneously.

Download Product Sheet

Unlock Single Cell Analysis with These System Features

  • Partition 500 – 80,000+ cells in under 9 minutes
  • Superior sensitivity and cell capture rate up to 65%
  • Simple workflow
  • Cell size flexibility, no lower limits
  • Low doublet rates of under 0.9% in 1,000 cells
View the Workflow
Single Cell Solution Family

Getting Started Guide:
Single Cell Gene Expression

Whether you have questions about how to design your experiments, optimize parameters, or identify the computational/analytical tools necessary to best analyze your single cell gene expression data, this guide has you covered. Take full advantage of the rich information enabled by single cell transcriptomic technology by downloading the getting started guide today!

Download Getting Started Guide

Make Every Cell Count

Watch How it Works

Analyze thousands of single cells in every run. See how the 10x technology suite performs millions of parallel reactions to enable gene expression profiling at scale with single cell resolution.


Feature Barcoding Technology

Watch How it Works

See how combining our solution with Feature Barcoding technology allows you to dramatically increase the understanding of complex biological systems. Watch examples of how you can get a more complete cellular read-out, cell by cell—the possibilities are virtually limitless.

Single Cell Controller

The Chromium Controller

Compact, Sleek, Efficient.

The compact, sleek Chromium Controller has been designed to rapidly and efficiently automate the equivalent of 100,000s to 1,000,000s of pipetting steps for highly parallel sample partitioning and molecular barcoding. The Chromium Controller allows a user to run any Chromium Solution, spanning from genome to single cell analysis. A dedicated Chromium Single Cell Controller is also available for users who exclusively run Single Cell Solutions.

View Our Controller Gallery

Product List

To view a list of products by application type, first select your desired version of the Single Cell Gene Expression Solution. If you would like to combine this solution with Feature Barcoding technology, select Feature Barcode type(s).

Gene Expression Version

Feature Barcode Selection

Reagents & Consumables

Reactions
Product Code
Chromium Chip B Single Cell Kit
1000074
1000073
Chromium i7 Multiplex Kit
96 rxns
120262
Chromium Single Cell 3′ Feature Barcode Library Kit
16 rxns
1000079
Chromium Single Cell 3′ Library & Gel Bead Kit v2
120267
120237
Chromium Single Cell 3′ Library & Gel Bead Kit v3
1000092
1000075
Chromium Single Cell A Chip Kit
1000009
120236

Compatible Partner Products

Biolegend TotalSeq™- A
Biolegend TotalSeq™- B

Instruments

Warranty
Product Code
Chromium Controller & Accessory Kit
120223
120246
Chromium Single Cell Controller & Accessory Kit
120263
120212

Software

Cell Ranger Analysis Pipelines
Loupe Cell Browser

Documentation

Demonstrated Protocol: Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
Technical Note: Guide RNA Specifications Compatible with Feature Barcoding Technology for CRISPR Screening
User Guide : Chromium Single Cell 3′ Reagent Kits (v3 Chemistry) with CRISPR Screening Feature Barcoding Technology
User Guide: Chromium Single Cell 3′ Reagent Kits (v3 Chemistry)
User Guide: Chromium Single Cell 3′ Reagent Kits (v3 Chemistry) with Cell Surface Protein Feature Barcoding Technology
User Guide: Chromium Single Cell 3′ Reagents v2
Single Cell Gene Expression Datasets
Scientific Seminars

Single Cell RNA Sequencing Unveils Mechanisms of Response and Resistance to Immunotherapy

 
  • Aude Chapuis
Fred Hutchinson Cancer Research Center
May 21, 2018
AACR

Single Cell RNA Sequencing Unveils Mechanisms of Response and Resistance to T-Cell Immunotherapy

 
  • Aude Chapuis
Fred Hutchinson Cancer Research Center
February 13, 2018
AGBT

Harnessing the Therapeutic Potential of Adoptively Transferred Cells

 
  • Aude Chapuis
Fred Hutchinson Cancer Research Center
June 22, 2017
Nature Webcast

Perturb-seq: High Throughput CRISPR Screening Via Single-Cell RNA Sequencing

 
  • Dr. Jonathan Weissman
University of California, San Francisco and Howard Hughes Medical Institute
May 10, 2017
Nature Webinar

Perturb-seq: Single-cell CRISPRi Screens Interrogate the Unfolded Protein Response

 
  • Britt Adamson
University of California, San Francisco
March 21, 2017
User Group Meeting - San Francisco, CA