Reveal the Full Complexity of Cellular Diversity with Single Cell Transcriptomics

Chromium Single Cell Gene Expression Solution

Go Beyond Traditional Gene Expression Analysis

The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to tens of thousands of cells. Our latest improvements allow you to detect even more unique transcripts per cell, and with the addition of Feature Barcoding technology, you can get a more complete molecular readout cell by cell—identify cell-specific CRISPR-mediated perturbations or simultaneously measure gene and cell surface protein expression in the same cell, with virtually unlimited possibilities for the additional feature types.

Go beyond traditional gene expression analysis to characterize cell populations, cell types, cell states, and more on a cell-by-cell basis. From assessing tumor heterogeneity and stem cell composition, to dissecting neuronal populations—the technological advancements provided by the Chromium Single Cell Gene Expression Solution, along with turnkey software tools, allow the creation of high complexity libraries from single cells to maximize insight from any sample type.

  • Identify and characterize rare cell types
  • Analyze and understand cellular heterogeneity and how this contributes to your biological system
  • Perform cellular phenotyping with single cell RNA-seq to identify novel targets, biomarkers, and cell types and states without the need for pre-selected targets
  • Evaluate mRNA and cell surface protein expression profiles within the same cell
  • Perform high-throughput and high-resolution functional genetic screens in tens of thousands of cells simultaneously
  • Assess comprehensive gene expression phenotypes for individual CRISPR perturbations
Gene Expression Profiling

Gene Expression Profiling

Uncover cell-to-cell gene expression variability and identify rare cell types in heterogeneous samples to better characterize cellular contributions during development and disease. Our latest improvements vastly increase sensitivity, so you can detect even more unique transcripts per cell.

Feature Barcoding Technology: Cell Surface Protein

Feature Barcoding Technology: Cell Surface Protein

Better implement published methods like CITE-Seq/REAP-Seq with our new and improved workflow using Feature Barcoding technology. Seamlessly measure both gene and cell surface protein expression in the same cell to identify protein isoforms, detect protein for low abundance transcripts, and further increase phenotypic specificity.

Antigen Specificity

Feature Barcoding Technology: CRISPR Screening

Identify CRISPR-mediated perturbations alongside cellular transcripts using Feature Barcoding technology. Implement this improved and novel methodology to conduct high-throughput and scalable functional genetic screens in hundreds to tens of thousands of single cells simultaneously.

Unlock Single Cell Analysis with These System Features

  • Partition 100 – 80,000+ cells efficiently
  • Superior sensitivity and cell capture rate up to 65%
  • Simple workflow
  • Cell size flexibility, no lower limits
  • Low doublet rates of under 0.9% in 1,000 cells
  • Available with Next GEM technology
View the Workflow
Single Cell Product Family

Getting Started Guide:
Single Cell Gene Expression

Whether you have questions about how to design your experiments, optimize parameters, or identify the computational/analytical tools necessary to best analyze your single cell gene expression data, this guide has you covered. Take full advantage of the rich information enabled by single cell transcriptomic technology by downloading the getting started guide today!

Download Getting Started Guide

Make Every Cell Count

Watch How it Works

Analyze thousands of single cells in every run. See how the 10x technology suite performs millions of parallel reactions to enable gene expression profiling at scale with single cell resolution.


Feature Barcoding Technology

Watch How it Works

See how combining our solution with Feature Barcoding technology allows you to dramatically increase the understanding of complex biological systems. Watch examples of how you can get a more complete cellular read-out, cell by cell—the possibilities are virtually limitless.

Single Cell Controller

Chromium Controller

Compact, Sleek, Efficient.

The compact, sleek Chromium Controller rapidly and efficiently combines large partition numbers with a massively diverse barcode library to generate >100,000 barcode-containing partitions in a matter of minutes. The Chromium Controller fits on a standard laboratory bench and allows a user to run any Chromium Solution, from genome to single cell analysis.

View Our Controller Gallery

Product List

To view a list of products by application type, first select your desired version of the Single Cell Gene Expression Solution. If you would like to combine this solution with Feature Barcoding technology, select Feature Barcode type(s).

Gene Expression Version

Feature Barcode Selection

Reagents & Consumables

Reactions
Product Code
Chromium Chip B Single Cell Kit
1000074
1000073
Chromium i7 Multiplex Kit
96 rxns
120262
Chromium Next GEM Chip G Single Cell Kit
1000127
1000120
Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1
1000128
1000121
Chromium Next GEM Single Cell 3’ Library Construction Kit v3.1
16 rxns
1000157
Chromium Single Cell 3′ Feature Barcode Library Kit
16 rxns
1000079
Chromium Single Cell 3′ Feature Barcode Library Kit
16 rxns
1000079
Chromium Single Cell 3′ Library & Gel Bead Kit v2
120267
120237
Chromium Single Cell 3′ Library & Gel Bead Kit v3
1000092
1000075
Chromium Single Cell 3′ Library Construction Kit v3
16 rxns
1000078
Chromium Single Cell A Chip Kit
1000009
120236
Single Index Kit T Set A
96 rxns
1000213

Compatible Partner Products

Biolegend TotalSeq™-A
Biolegend TotalSeq™-B

Instruments

Warranty
Product Code
Chromium Controller & Next GEM Accessory Kit
120223
120246
Chromium Controller & Next GEM Accessory Kit
1000202
1000204

Software

Cell Ranger Analysis Pipelines
Loupe Browser

Documentation

Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry)
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for CRISPR Screening
Demonstrated Protocol: Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
Technical Note: Guide RNA Specifications Compatible with Feature Barcoding Technology for CRISPR Screening
User Guide : Chromium Single Cell 3′ Reagent Kits (v3 Chemistry) with CRISPR Screening Feature Barcoding Technology
User Guide: Chromium Single Cell 3′ Reagent Kits (v3 Chemistry)
User Guide: Chromium Single Cell 3′ Reagent Kits (v3 Chemistry) with Cell Surface Protein Feature Barcoding Technology
User Guide: Chromium Single Cell 3′ Reagents v2
Single Cell Gene Expression Datasets
Scientific Seminars

de Novo Assembly of a Large Size Genome: the Olive Fruit Fly

 
  • Jiannis Ragoussis
McGill University
February 14, 2017
AGBT Annual Meeting – Hollywood, FL

Spanish Webinar – Biology at True Resolution

 
  • Luca Mazzitelli
  • Pernille Albertus
  • Roser Vento-Tormo
10x Genomics
July 16, 2019

Visualizing and Exploring Single Cell Gene Expression Data

 
  • Juan Du, PhD
Software Field Operations Engineer, 10x Genomics
August 30, 2019
Poster Hall

High throughput immune profiling using integrated single cell multi-omics analyses

  • Single Cell Biology Keystone Symposia
    Breckenridge, CO
    January 2019

Single cell ATAC-seq for characterization of complex biological systems

  • Single Cell Biology Keystone Symposia
    Breckenridge, CO
    January 2019

Single cell ATAC-seq for characterization of complex biological systems

  • Human Cell Atlas Conference
    Cambridge, MA
    November 2018

Order your 10x system, today.