Visium Spatial Gene Expression
Map the Whole Transcriptome Within the Tissue Context
Visium Spatial Gene Expression is a next-generation molecular technology for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context to discover novel insights into normal development, disease pathology, and clinical translational research.
Combine whole transcriptome spatial analysis with immunofluorescence protein detection.
Profile entire tissue sections
No need to select regions of interest—analyze the whole transcriptome from an entire section.
1–10 cell resolution on average per spot depending on tissue type.
Streamlined data analysis
Combine histological and gene expression data with easy-to-use software.
One-day lab workflow
From tissue section to sequencing-ready library in one day.
Diverse sample compatibility
Demonstrated on a diverse set of organs across species (human, mouse, rat, and more).
Explore what you can do
Gain a complete view of disease complexity
Discover new biomarkers and identify novel cell types and states
Map the spatial organization of cell atlases
Identify spatiotemporal gene expression patterns
Flexible Targeted and Whole Transcriptome Assays
Spatially map global gene expression with whole transcriptome analysis or focus on your genes of interest using targeted gene expression panels.
Targeted Gene Expression Panels (Coming soon)
Choose from pre-designed cancer, immunology, neuroscience and gene signature panels with the ability to add up to 200 custom genes, or design fully custom panels. Optimized protocol, support, and software for targeted panels with Visium Spatial Gene Expression coming September 2020.
With our comprehensive Visium Spatial Gene Expression solutions you get:
Envision the spatial organization of newly discovered cell types, states, and biomarkers with whole transcriptome analysis.
Transition seamlessly between whole transcriptome and targeted analysis from the same library preparation to validate discoveries and explore new hypotheses.
Scale up from a few genes of interest to all relevant genes and pathways by combining high-throughput spatial gene expression with the efficiency of targeted panels.
Extendable for Protein Co-detection
Enable discovery of novel cell types and their spatial organization by combining immunofluorescence and whole transcriptome spatial gene expression.
- Explore transcriptome and protein relationships with simultaneous spatial detection
- Discover new tissue biomarkers with cell type specificity
Our End-to-End Solution
Visium Spatial Tissue Optimization Slides
Easily adoptable within existing lab infrastructure, no need for a new instrument.
Visium Spatial Gene Expression Reagents
With our reagent kits, capture whole transcriptome gene expression with spatial resolution across an entire tissue section.
Analysis and Visualization Software
Our analysis pipelines
Our visualization software
World-Class Technical and Customer Support
Our expert support team can be contacted by phone or email.
Prepare your sample
Embed, section, and place fresh-frozen tissue onto a capture area of the gene expression slide. Each capture area has thousands of barcoded spots, each containing millions of capture oligonucleotides with spatial barcodes unique to that spot.Resources
Stain and image the tissue
Utilize standard fixation and staining techniques, including Hematoxylin and Eosin (H&E) staining, to visualize tissue sections on slides using a brightfield microscope and immunofluorescence staining to visualize protein detection in tissue sections on slides using a fluorescent microscope.Resources
Permeabilize tissue and construct library
Permeabilize the tissue to release mRNA from the cells, which binds to the spatially-barcoded oligonucleotides present on the spots. A reverse transcription reaction produces cDNA from the captured mRNA. The barcoded cDNA is then pooled for downstream processing to complete a sequencing-ready library.Resources
The resulting 10x Barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers for massive transcriptional profiling of entire tissue sections.Resources
Analyze and visualize your data
Use our Space Ranger analysis software to process your Spatial Gene Expression data and interactively explore the results with our Loupe Browser visualization software.
Do I need to be a bioinformatician to use Loupe?
Loupe is a point-and-click software that’s easy for anyone to download and use.Resources